Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1827933..1828523 Replicon chromosome
Accession NC_014618
Organism Enterobacter lignolyticus SCF1

Toxin (Protein)


Gene name pemK Uniprot ID E3G149
Locus tag ENTCL_RS08480 Protein ID WP_013365706.1
Coordinates 1828191..1828523 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID E3G148
Locus tag ENTCL_RS08475 Protein ID WP_013365705.1
Coordinates 1827933..1828190 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENTCL_RS08470 1827184..1827648 + 465 WP_013365704.1 hypothetical protein -
ENTCL_RS08475 1827933..1828190 + 258 WP_013365705.1 antitoxin Antitoxin
ENTCL_RS08480 1828191..1828523 + 333 WP_013365706.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ENTCL_RS08490 1828825..1830279 + 1455 WP_157865535.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ENTCL_RS08495 1830332..1831612 - 1281 WP_013365708.1 FAD-binding oxidoreductase -
ENTCL_RS08500 1831615..1833102 - 1488 WP_013365709.1 aldehyde dehydrogenase PuuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1820447..1828523 8076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11757.60 Da        Isoelectric Point: 10.4487

>T24937 WP_013365706.1 NC_014618:1828191-1828523 [[Enterobacter] lignolyticus SCF1]
MERGEVWLVSLDPTAGHEQSGKRPVLIVSPAAFNQITRLPVVLPITTGGNFARTAGFAVSLDGAGTKTTGVIRCDQPRTI
DMAARHGKRLERLPDGIINDVLARLATILT

Download         Length: 333 bp

>T24937 NC_014618:1828191-1828523 [[Enterobacter] lignolyticus SCF1]
ATGGAGAGAGGAGAGGTCTGGCTCGTTTCGCTTGATCCTACCGCCGGACATGAGCAAAGCGGAAAACGCCCGGTGCTTAT
CGTTTCTCCGGCTGCGTTTAACCAAATCACGCGGTTACCCGTGGTTCTTCCGATAACAACGGGTGGGAACTTTGCCCGTA
CTGCGGGGTTCGCCGTGTCTCTGGATGGGGCGGGAACGAAAACAACGGGCGTCATTCGCTGCGATCAGCCGAGAACCATT
GATATGGCGGCCAGACACGGTAAGCGGCTTGAGCGTCTGCCTGACGGGATCATCAACGACGTGCTGGCAAGACTGGCGAC
CATCCTGACCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9282.67 Da        Isoelectric Point: 4.4012

>AT24937 WP_013365705.1 NC_014618:1827933-1828190 [[Enterobacter] lignolyticus SCF1]
MYTTRLKKVGGSVMLAVPPAVLKTLALSADSEVGMTIDNGRIIIEPQKRPQYSLAELLAQCDPQAEISDEEREWVDSPAA
GRELL

Download         Length: 258 bp

>AT24937 NC_014618:1827933-1828190 [[Enterobacter] lignolyticus SCF1]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCGTCATGCTGGCCGTCCCGCCCGCCGTGCTGAAAACGCTGGCGCT
GTCGGCAGACAGCGAAGTCGGCATGACCATTGATAATGGTCGCATCATTATTGAACCGCAAAAGCGGCCTCAGTATTCGC
TGGCGGAGCTGCTGGCGCAGTGCGATCCGCAGGCTGAAATCAGCGATGAAGAGCGAGAATGGGTTGACTCGCCTGCCGCC
GGGAGGGAGCTTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E3G149

Antitoxin

Source ID Structure
AlphaFold DB A0A806X5S9

References