Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4632513..4633323 Replicon chromosome
Accession NZ_CP099743
Organism Klebsiella pneumoniae strain 503666-2-2019

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NHF44_RS22450 Protein ID WP_004178461.1
Coordinates 4632513..4633046 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NHF44_RS22455 Protein ID WP_002887278.1
Coordinates 4633057..4633323 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF44_RS22445 (NHF44_22430) 4631344..4632465 + 1122 WP_260565887.1 cupin domain-containing protein -
NHF44_RS22450 (NHF44_22435) 4632513..4633046 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NHF44_RS22455 (NHF44_22440) 4633057..4633323 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NHF44_RS22460 (NHF44_22445) 4633426..4634859 - 1434 WP_116954916.1 Cu(+)/Ag(+) sensor histidine kinase -
NHF44_RS22465 (NHF44_22450) 4634849..4635532 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NHF44_RS22470 (NHF44_22455) 4635704..4637089 + 1386 WP_032417819.1 efflux transporter outer membrane subunit -
NHF44_RS22475 (NHF44_22460) 4637107..4637451 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T249367 WP_004178461.1 NZ_CP099743:c4633046-4632513 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT249367 WP_002887278.1 NZ_CP099743:c4633323-4633057 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References