Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 181242..181885 Replicon plasmid pGDE043-2
Accession NZ_CP099723
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag NH569_RS26520 Protein ID WP_001034044.1
Coordinates 181469..181885 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag NH569_RS26515 Protein ID WP_001261286.1
Coordinates 181242..181472 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS26500 (176379) 176379..176609 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
NH569_RS26505 (176606) 176606..177022 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
NH569_RS26510 (177067) 177067..180861 - 3795 WP_001144732.1 hypothetical protein -
NH569_RS26515 (181242) 181242..181472 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NH569_RS26520 (181469) 181469..181885 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
NH569_RS26525 (181960) 181960..183525 + 1566 WP_001128474.1 AAA family ATPase -
NH569_RS26530 (183510) 183510..184532 + 1023 WP_032174242.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaLAP-2 / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..203860 203860
- flank IS/Tn - - 184786..185289 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T249346 WP_001034044.1 NZ_CP099723:181469-181885 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT249346 WP_001261286.1 NZ_CP099723:181242-181472 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References