Detailed information of TA system TA024934

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 723982..724661
• Replicon: chromosome
• Accession: NC_014618
• Organism: Enterobacter lignolyticus SCF1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E3G2M9
• Locus tag: ENTCL_RS22615
• Protein ID: WP_013364721.1
• Coordinates: 724320..724661 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: E3G2M8
• Locus tag: ENTCL_RS03465
• Protein ID: WP_013364720.1
• Coordinates: 723982..724299 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTCL_RS03435	719589..720416	+	828	WP_013364713.1	DUF945 domain-containing protein	-
ENTCL_RS03440	720643..721353	+	711	WP_013364714.1	transcriptional regulator	-
ENTCL_RS03445	721640..722509	+	870	WP_013364716.1	hypothetical protein	-
ENTCL_RS03450	722784..723242	+	459	WP_013364717.1	antirestriction protein	-
ENTCL_RS03455	723251..723733	+	483	WP_013364718.1	DNA repair protein RadC	-
ENTCL_RS03460	723742..723963	+	222	WP_013364719.1	DUF987 domain-containing protein	-
ENTCL_RS03465	723982..724299	+	318	WP_013364720.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ENTCL_RS22615	724320..724661	+	342	WP_013364721.1	TA system toxin CbtA family protein	Toxin
ENTCL_RS03475	724777..725610	+	834	WP_013364722.1	DUF4942 domain-containing protein	-
ENTCL_RS03485	725975..726481	+	507	WP_013364723.1	G/U mismatch-specific DNA glycosylase	-
ENTCL_RS03490	726532..728376	-	1845	WP_013364724.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	691168..767862	76694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-103)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12832.94 Da
• Isoelectric Point: 9.1484

>T24934 WP_013364721.1 NC_014618:724320-724661 [[Enterobacter] lignolyticus SCF1]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
IWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Nucleotide

• Length: 342 bp

>T24934 NC_014618:724320-724661 [[Enterobacter] lignolyticus SCF1]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
ATCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11885.43 Da
• Isoelectric Point: 6.6246

>AT24934 WP_013364720.1 NC_014618:723982-724299 [[Enterobacter] lignolyticus SCF1]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Nucleotide

• Length: 318 bp

>AT24934 NC_014618:723982-724299 [[Enterobacter] lignolyticus SCF1]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGCAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCTGTTTACCCCATCCAACGATAA

Structures

Toxin

• AlphaFold DB: E3G2M9

Antitoxin

• AlphaFold DB: E3G2M8