Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86689..88312 Replicon plasmid pGDE043-mcr1
Accession NZ_CP099722
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NH569_RS24570 Protein ID WP_000088046.1
Coordinates 86987..88312 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NH569_RS24565 Protein ID WP_024136327.1
Coordinates 86689..86982 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS24540 (NH569_24540) 82561..83802 + 1242 WP_001053338.1 VWA domain-containing protein -
NH569_RS24545 (NH569_24545) 84365..84646 - 282 WP_000077926.1 hypothetical protein -
NH569_RS24550 (NH569_24550) 84696..84887 - 192 WP_000398480.1 hypothetical protein -
NH569_RS24555 (NH569_24555) 84979..85320 - 342 WP_001151575.1 hypothetical protein -
NH569_RS24560 (NH569_24560) 85693..86085 + 393 WP_000341066.1 hypothetical protein -
NH569_RS24565 (NH569_24565) 86689..86982 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NH569_RS24570 (NH569_24570) 86987..88312 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NH569_RS24575 (NH569_24575) 88373..88579 + 207 WP_000134171.1 hypothetical protein -
NH569_RS24580 (NH569_24580) 88681..89091 + 411 WP_000985911.1 hypothetical protein -
NH569_RS24585 (NH569_24585) 89104..89919 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
NH569_RS24590 (NH569_24590) 90173..90598 + 426 WP_001043843.1 hypothetical protein -
NH569_RS24595 (NH569_24595) 91147..91455 + 309 WP_001224687.1 hypothetical protein -
NH569_RS24600 (NH569_24600) 91471..92328 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / mph(A) / tet(M) / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / ant(3'')-Ia / lnu(F) / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / floR / blaCTX-M-65 / oqxA / oqxB - 1..260179 260179


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T249338 WP_000088046.1 NZ_CP099722:86987-88312 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT249338 WP_024136327.1 NZ_CP099722:86689-86982 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References