Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4401254..4401751 Replicon chromosome
Accession NZ_CP099721
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NH569_RS21700 Protein ID WP_001173343.1
Coordinates 4401254..4401427 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag NH569_RS21705 Protein ID WP_001312331.1
Coordinates 4401455..4401751 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS21690 (4397924) 4397924..4399381 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
NH569_RS21695 (4399618) 4399618..4401135 + 1518 WP_000003806.1 lysine--tRNA ligase -
NH569_RS21700 (4401254) 4401254..4401427 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NH569_RS21705 (4401455) 4401455..4401751 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NH569_RS21710 (4401979) 4401979..4402251 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NH569_RS21715 (4402263) 4402263..4402493 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
NH569_RS21720 (4402674) 4402674..4404305 + 1632 WP_001216482.1 sensor histidine kinase -
NH569_RS21725 (4404302) 4404302..4405021 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4391005..4401751 10746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T249336 WP_001173343.1 NZ_CP099721:c4401427-4401254 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT249336 WP_001312331.1 NZ_CP099721:c4401751-4401455 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References