Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1164276..1164859 Replicon chromosome
Accession NZ_CP099721
Organism Escherichia coli strain EC21GDE043

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag NH569_RS05650 Protein ID WP_000254750.1
Coordinates 1164524..1164859 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NH569_RS05645 Protein ID WP_000581937.1
Coordinates 1164276..1164524 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NH569_RS05635 (1160615) 1160615..1161916 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
NH569_RS05640 (1161964) 1161964..1164198 + 2235 WP_000226815.1 GTP pyrophosphokinase -
NH569_RS05645 (1164276) 1164276..1164524 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NH569_RS05650 (1164524) 1164524..1164859 + 336 WP_000254750.1 endoribonuclease MazF Toxin
NH569_RS05655 (1164931) 1164931..1165722 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NH569_RS05660 (1165950) 1165950..1167587 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NH569_RS05665 (1167675) 1167675..1168973 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T249318 WP_000254750.1 NZ_CP099721:1164524-1164859 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT249318 WP_000581937.1 NZ_CP099721:1164276-1164524 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References