Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 3502366..3502968 Replicon chromosome
Accession NZ_CP099629
Organism Paraburkholderia fungorum strain OTU2BAGNBA2

Toxin (Protein)


Gene name PumA Uniprot ID A0A2N8Q3W2
Locus tag NFS19_RS38360 Protein ID WP_028194450.1
Coordinates 3502366..3502665 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0D5VNN5
Locus tag NFS19_RS38365 Protein ID WP_028194449.1
Coordinates 3502669..3502968 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFS19_RS38345 (NFS19_38335) 3498668..3499621 - 954 WP_235430069.1 choline ABC transporter substrate-binding protein -
NFS19_RS38350 (NFS19_38340) 3499673..3501220 - 1548 WP_252890181.1 choline-sulfatase -
NFS19_RS38355 (NFS19_38345) 3501373..3502287 + 915 WP_178370025.1 LysR family transcriptional regulator -
NFS19_RS38360 (NFS19_38350) 3502366..3502665 + 300 WP_028194450.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NFS19_RS38365 (NFS19_38355) 3502669..3502968 + 300 WP_028194449.1 putative addiction module antidote protein Antitoxin
NFS19_RS38370 (NFS19_38360) 3503140..3504015 + 876 WP_252890180.1 formyltetrahydrofolate deformylase -
NFS19_RS38375 (NFS19_38365) 3504110..3504967 - 858 WP_028194447.1 glycine betaine ABC transporter substrate-binding protein -
NFS19_RS38380 (NFS19_38370) 3505110..3506366 - 1257 WP_102857553.1 hybrid-cluster NAD(P)-dependent oxidoreductase -
NFS19_RS38385 (NFS19_38375) 3506403..3507713 - 1311 WP_046570534.1 aromatic ring-hydroxylating dioxygenase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3501373..3506366 4993
- inside Prophage - - 3501373..3516634 15261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-87)

Antitoxin

Cro/C1-type helix-turn-helix domain

(48-92)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11009.69 Da        Isoelectric Point: 9.6247

>T249279 WP_028194450.1 NZ_CP099629:3502366-3502665 [Paraburkholderia fungorum]
MLKIRTTETFDAWFDGLKDRIARRRIQARIDRLAMGNPGDTNSAGAPIIELRIDHGPGYRVYYVQRGAVLVILLCGGDKS
TQPADIKAAHKMLANLESE

Download         Length: 300 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10603.11 Da        Isoelectric Point: 6.0723

>AT249279 WP_028194449.1 NZ_CP099629:3502669-3502968 [Paraburkholderia fungorum]
MGKIKTRPWDSAEHLKTEADMAEYFEACLVEGGDDPAFLAHALGVIARARGMAQLARDTGISREGLYKALSEDGNPSFAT
IIKVIRALGLELHAAPAHA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8Q3W2

Antitoxin

Source ID Structure
AlphaFold DB A0A0D5VNN5

References