Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 5851156..5852780 Replicon chromosome
Accession NZ_CP099600
Organism Pseudomonas siliginis strain OTU6MEDAA1

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag NF678_RS26110 Protein ID WP_252882889.1
Coordinates 5851431..5852780 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A554AI87
Locus tag NF678_RS26105 Protein ID WP_041476582.1
Coordinates 5851156..5851434 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NF678_RS26075 (NF678_26075) 5846591..5847214 + 624 WP_236167796.1 FMN-binding negative transcriptional regulator -
NF678_RS26080 (NF678_26080) 5847225..5847671 + 447 WP_236167797.1 GNAT family N-acetyltransferase -
NF678_RS26085 (NF678_26085) 5847684..5848355 + 672 WP_252882887.1 GNAT family protein -
NF678_RS26090 (NF678_26090) 5848478..5849380 - 903 WP_252882888.1 homocysteine S-methyltransferase family protein -
NF678_RS26095 (NF678_26095) 5849494..5850315 + 822 WP_076564634.1 ABC transporter substrate-binding protein -
NF678_RS26100 (NF678_26100) 5850312..5851148 + 837 WP_122698746.1 amino acid ABC transporter permease -
NF678_RS26105 (NF678_26105) 5851156..5851434 - 279 WP_041476582.1 helix-turn-helix transcriptional regulator Antitoxin
NF678_RS26110 (NF678_26110) 5851431..5852780 - 1350 WP_252882889.1 HipA domain-containing protein Toxin
NF678_RS26115 (NF678_26115) 5852809..5853093 - 285 WP_252882890.1 hypothetical protein -
NF678_RS26120 (NF678_26120) 5853150..5855339 + 2190 WP_252882891.1 autotransporter outer membrane beta-barrel domain-containing protein -
NF678_RS26125 (NF678_26125) 5855549..5857357 - 1809 WP_083353857.1 sodium-extruding oxaloacetate decarboxylase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(165-367)

Antitoxin

Cro/C1-type helix-turn-helix domain

(27-80)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 49714.34 Da        Isoelectric Point: 5.1471

>T249220 WP_252882889.1 NZ_CP099600:c5852780-5851431 [Pseudomonas siliginis]
MYNLTLQIFTAGQWLDAMILDFDDPEKGFESRCSFGYEADYLVENIELIGSPFSQAVSALYPLDWEGRRAKTPAFVHDIA
PAGAAKRFLLARLGQERPAEISADLFLLGRSTPAPIGNMRIKESAEAVDDRKPIGFKREEVISRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENSAGLLYPDAVLDDEEVRQHWFVKFARNRGGQTDQDILRSEFHYYKALQTLGIATVAAEGLALE
EATKPSLWMRRFDRQVKDGAVSRFAVESIYSLAEVTTPGSAMNHLEVIRMLAGLWRKAGQASQIPGLVADYLRRDLINKI
LGNSDNHGRNTAIIRQENAFHLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRVLADSADPHDSLAGLDASA
LFEGLHADARRLAALPDILAASGLPARTMNHPSVHLKNLEQRLKEWDLQ

Download         Length: 1350 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10332.12 Da        Isoelectric Point: 10.7364

>AT249220 WP_041476582.1 NZ_CP099600:c5851434-5851156 [Pseudomonas siliginis]
MSLTVLERTALIESIQQSLAEGTLEIGDAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDTI

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A554AI87

References