Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1369273..1370189 | Replicon | chromosome |
| Accession | NC_014551 | ||
| Organism | Bacillus amyloliquefaciens DSM 7 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | A0A7U5AVM4 |
| Locus tag | BAMF_RS27510 | Protein ID | WP_013351967.1 |
| Coordinates | 1369443..1370189 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | I2HQ14 |
| Locus tag | BAMF_RS27505 | Protein ID | WP_003154807.1 |
| Coordinates | 1369273..1369443 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BAMF_RS41420 | 1364552..1365181 | + | 630 | Protein_1384 | hypothetical protein | - |
| BAMF_RS41240 | 1365665..1366126 | + | 462 | WP_127721186.1 | hypothetical protein | - |
| BAMF_RS27475 | 1366139..1366537 | + | 399 | WP_013351961.1 | hypothetical protein | - |
| BAMF_RS27480 | 1366524..1366721 | + | 198 | WP_013351962.1 | XkdX family protein | - |
| BAMF_RS27485 | 1366770..1367531 | + | 762 | WP_013351963.1 | hypothetical protein | - |
| BAMF_RS27490 | 1367585..1367848 | + | 264 | WP_013351964.1 | hemolysin XhlA family protein | - |
| BAMF_RS27495 | 1367862..1368125 | + | 264 | WP_013351965.1 | phage holin | - |
| BAMF_RS27500 | 1368139..1369017 | + | 879 | WP_013351966.1 | N-acetylmuramoyl-L-alanine amidase | - |
| BAMF_RS27505 | 1369273..1369443 | - | 171 | WP_003154807.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| BAMF_RS27510 | 1369443..1370189 | - | 747 | WP_013351967.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| BAMF_RS27515 | 1370297..1371295 | - | 999 | WP_013351968.1 | inorganic phosphate transporter | - |
| BAMF_RS27520 | 1371308..1371925 | - | 618 | WP_003154804.1 | DUF47 domain-containing protein | - |
| BAMF_RS27525 | 1372210..1373526 | - | 1317 | WP_013351969.1 | amino acid permease | - |
| BAMF_RS27530 | 1373849..1374799 | + | 951 | WP_013351970.1 | ring-cleaving dioxygenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29054.53 Da Isoelectric Point: 4.6839
>T24918 WP_013351967.1 NC_014551:c1370189-1369443 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG
Download Length: 747 bp
>T24918 NC_014551:c1370189-1369443 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6676.74 Da Isoelectric Point: 10.9273
>AT24918 WP_003154807.1 NC_014551:c1369443-1369273 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA
Download Length: 171 bp
>AT24918 NC_014551:c1369443-1369273 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U5AVM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I2HQ14 |