Detailed information of TA system TA024917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 480337..480974
• Replicon: chromosome
• Accession: NC_014551
• Organism: Bacillus amyloliquefaciens DSM 7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAMF_RS22640
• Protein ID: WP_003156187.1
• Coordinates: 480624..480974 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAMF_RS22635
• Protein ID: WP_003156188.1
• Coordinates: 480337..480618 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMF_RS22615	476703..477302	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
BAMF_RS22620	477395..477760	+	366	WP_013351093.1	holo-ACP synthase	-
BAMF_RS22625	477925..478932	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
BAMF_RS22630	479049..480218	+	1170	WP_013351095.1	alanine racemase	-
BAMF_RS22635	480337..480618	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAMF_RS22640	480624..480974	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAMF_RS22645	481095..481916	+	822	WP_013351096.1	STAS domain-containing protein	-
BAMF_RS22650	481921..482286	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAMF_RS22655	482289..482690	+	402	WP_013351097.1	anti-sigma regulatory factor	-
BAMF_RS22660	482702..483709	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
BAMF_RS22665	483773..484102	+	330	WP_013351099.1	anti-sigma factor antagonist	-
BAMF_RS22670	484099..484581	+	483	WP_013351100.1	anti-sigma B factor RsbW	-
BAMF_RS22675	484547..485335	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
BAMF_RS22680	485335..485937	+	603	WP_013351102.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T24917 WP_003156187.1 NC_014551:480624-480974 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T24917 NC_014551:480624-480974 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT24917 WP_003156188.1 NC_014551:480337-480618 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT24917 NC_014551:480337-480618 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5