Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 480337..480974 Replicon chromosome
Accession NC_014551
Organism Bacillus amyloliquefaciens DSM 7

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAMF_RS22640 Protein ID WP_003156187.1
Coordinates 480624..480974 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAMF_RS22635 Protein ID WP_003156188.1
Coordinates 480337..480618 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAMF_RS22615 476703..477302 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
BAMF_RS22620 477395..477760 + 366 WP_013351093.1 holo-ACP synthase -
BAMF_RS22625 477925..478932 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
BAMF_RS22630 479049..480218 + 1170 WP_013351095.1 alanine racemase -
BAMF_RS22635 480337..480618 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAMF_RS22640 480624..480974 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAMF_RS22645 481095..481916 + 822 WP_013351096.1 STAS domain-containing protein -
BAMF_RS22650 481921..482286 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAMF_RS22655 482289..482690 + 402 WP_013351097.1 anti-sigma regulatory factor -
BAMF_RS22660 482702..483709 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
BAMF_RS22665 483773..484102 + 330 WP_013351099.1 anti-sigma factor antagonist -
BAMF_RS22670 484099..484581 + 483 WP_013351100.1 anti-sigma B factor RsbW -
BAMF_RS22675 484547..485335 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
BAMF_RS22680 485335..485937 + 603 WP_013351102.1 SpoIIE family protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T24917 WP_003156187.1 NC_014551:480624-480974 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T24917 NC_014551:480624-480974 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT24917 WP_003156188.1 NC_014551:480337-480618 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT24917 NC_014551:480337-480618 [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References