249149

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3653245..3653924 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag NHF38_RS17915 Protein ID WP_000854672.1
Coordinates 3653583..3653924 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag NHF38_RS17910 Protein ID WP_000070395.1
Coordinates 3653245..3653562 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS17860 (3648292) 3648292..3649155 + 864 WP_001065553.1 GTPase family protein -
NHF38_RS17865 (3649247) 3649247..3650068 + 822 WP_000197389.1 DUF932 domain-containing protein -
NHF38_RS17870 (3650285) 3650285..3650476 + 192 Protein_3518 DeoR family transcriptional regulator -
NHF38_RS17875 (3650472) 3650472..3650699 + 228 WP_001548158.1 protein YpjK -
NHF38_RS17880 (3650699) 3650699..3651142 + 444 WP_000824223.1 lipoprotein YafY -
NHF38_RS17885 (3651165) 3651165..3651632 + 468 WP_001547765.1 protein YkfB -
NHF38_RS17890 (3651709) 3651709..3651948 + 240 WP_000194654.1 DUF905 family protein -
NHF38_RS17895 (3652046) 3652046..3652504 + 459 WP_000211838.1 antirestriction protein -
NHF38_RS17900 (3652520) 3652520..3652996 + 477 WP_000811693.1 RadC family protein -
NHF38_RS17905 (3653005) 3653005..3653226 + 222 WP_000691994.1 DUF987 domain-containing protein -
NHF38_RS17910 (3653245) 3653245..3653562 + 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
NHF38_RS17915 (3653583) 3653583..3653924 + 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
NHF38_RS17925 (3654496) 3654496..3655749 - 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
NHF38_RS17930 (3655761) 3655761..3656864 - 1104 WP_001285288.1 glutamate 5-kinase -
NHF38_RS17935 (3657152) 3657152..3658207 + 1056 WP_000749863.1 phosphoporin PhoE -
NHF38_RS17940 (3658246) 3658246..3658647 - 402 WP_000174689.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T249149 WP_000854672.1 NZ_CP099588:3653583-3653924 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT249149 WP_000070395.1 NZ_CP099588:3653245-3653562 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References