Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 843356..844010 Replicon chromosome
Accession NZ_CP099588
Organism Escherichia coli strain EMG2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NHF38_RS04090 Protein ID WP_000244777.1
Coordinates 843603..844010 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NHF38_RS04085 Protein ID WP_000354046.1
Coordinates 843356..843622 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHF38_RS04060 (838525) 838525..839268 + 744 WP_000951964.1 SDR family oxidoreductase -
NHF38_RS04065 (839325) 839325..840758 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NHF38_RS04070 (840803) 840803..841114 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NHF38_RS04075 (841278) 841278..841937 + 660 WP_000250274.1 hemolysin III family protein -
NHF38_RS04080 (842133) 842133..843113 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NHF38_RS04085 (843356) 843356..843622 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NHF38_RS04090 (843603) 843603..844010 + 408 WP_000244777.1 protein YgfX Toxin
NHF38_RS04095 (844050) 844050..844571 - 522 WP_001055874.1 flavodoxin FldB -
NHF38_RS04100 (844683) 844683..845579 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NHF38_RS04105 (845604) 845604..846314 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NHF38_RS04110 (846320) 846320..848053 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T249135 WP_000244777.1 NZ_CP099588:843603-844010 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp


Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT249135 WP_000354046.1 NZ_CP099588:843356-843622 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References