Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 185883..186409 Replicon plasmid pSZS128-Hv-MDR
Accession NZ_CP099523
Organism Klebsiella pneumoniae strain SZS128

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NG894_RS27450 Protein ID WP_000323025.1
Coordinates 185883..186170 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NG894_RS27455 Protein ID WP_000534858.1
Coordinates 186170..186409 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NG894_RS27420 (NG894_27405) 180929..181162 - 234 WP_004181905.1 hypothetical protein -
NG894_RS27425 (NG894_27410) 181241..181588 - 348 WP_004181904.1 hypothetical protein -
NG894_RS27430 (NG894_27415) 181744..182811 - 1068 WP_004181903.1 hypothetical protein -
NG894_RS27435 (NG894_27420) 183501..183878 + 378 WP_004181901.1 hypothetical protein -
NG894_RS27440 (NG894_27425) 184076..185050 - 975 WP_004181899.1 hypothetical protein -
NG894_RS27445 (NG894_27430) 185653..185811 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
NG894_RS27450 (NG894_27435) 185883..186170 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NG894_RS27455 (NG894_27440) 186170..186409 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NG894_RS27460 (NG894_27445) 186434..186538 + 105 Protein_201 hypothetical protein -
NG894_RS27465 (NG894_27450) 186672..187088 - 417 Protein_202 cation-efflux pump -
NG894_RS27470 (NG894_27455) 187161..188329 + 1169 WP_085929673.1 IS3-like element ISEc52 family transposase -
NG894_RS27475 (NG894_27460) 188339..188650 - 312 Protein_204 IS110 family transposase -
NG894_RS27480 (NG894_27465) 189071..190411 - 1341 WP_000589001.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IId / blaSHV-12 / qnrB4 / blaDHA-1 / sul1 / armA / msr(E) / mph(E) / mph(A) rmpA / iutA / iucD / iucC / iucB / iucA 1..302470 302470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T249072 WP_000323025.1 NZ_CP099523:c186170-185883 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT249072 WP_000534858.1 NZ_CP099523:c186409-186170 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References