Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4721851..4722661 Replicon chromosome
Accession NZ_CP099522
Organism Klebsiella pneumoniae strain SZS128

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag NG894_RS23360 Protein ID WP_002887280.1
Coordinates 4721851..4722384 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NG894_RS23365 Protein ID WP_002887278.1
Coordinates 4722395..4722661 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NG894_RS23355 (NG894_23345) 4720682..4721803 + 1122 WP_002887282.1 cupin domain-containing protein -
NG894_RS23360 (NG894_23350) 4721851..4722384 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
NG894_RS23365 (NG894_23355) 4722395..4722661 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NG894_RS23370 (NG894_23360) 4722764..4724197 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
NG894_RS23375 (NG894_23365) 4724187..4724870 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NG894_RS23380 (NG894_23370) 4725042..4726427 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
NG894_RS23385 (NG894_23375) 4726445..4726789 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T249066 WP_002887280.1 NZ_CP099522:c4722384-4721851 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT249066 WP_002887278.1 NZ_CP099522:c4722661-4722395 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References