Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 706064..706839 Replicon chromosome
Accession NZ_CP099522
Organism Klebsiella pneumoniae strain SZS128

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NG894_RS03540 Protein ID WP_004150910.1
Coordinates 706354..706839 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NG894_RS03535 Protein ID WP_004150912.1
Coordinates 706064..706357 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NG894_RS03515 (NG894_03515) 701195..702106 + 912 WP_004188550.1 LysR family transcriptional regulator -
NG894_RS03520 (NG894_03520) 702107..703255 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NG894_RS03525 (NG894_03525) 703266..704642 - 1377 WP_004217775.1 cystathionine beta-synthase -
NG894_RS03535 (NG894_03535) 706064..706357 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NG894_RS03540 (NG894_03540) 706354..706839 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NG894_RS03545 (NG894_03545) 707543..708136 + 594 WP_133400560.1 hypothetical protein -
NG894_RS03550 (NG894_03550) 708233..708449 + 217 Protein_697 transposase -
NG894_RS03555 (NG894_03555) 709055..709927 + 873 WP_004188557.1 ParA family protein -
NG894_RS03560 (NG894_03560) 709927..710310 + 384 WP_004150906.1 hypothetical protein -
NG894_RS03565 (NG894_03565) 710303..711670 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 705021..708385 3364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T249058 WP_004150910.1 NZ_CP099522:706354-706839 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT249058 WP_004150912.1 NZ_CP099522:706064-706357 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References