Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1249192..1250109 Replicon chromosome
Accession NZ_CP099465
Organism Bacillus velezensis strain Ag75

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag NG745_RS06640 Protein ID WP_032866111.1
Coordinates 1249363..1250109 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag NG745_RS06635 Protein ID WP_003154807.1
Coordinates 1249192..1249362 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NG745_RS06595 (NG745_06595) 1244417..1246048 + 1632 WP_060561803.1 pyocin knob domain-containing protein -
NG745_RS06600 (NG745_06600) 1246061..1246432 + 372 WP_060561802.1 XkdW family protein -
NG745_RS06605 (NG745_06605) 1246437..1246634 + 198 WP_059367156.1 XkdX family protein -
NG745_RS06610 (NG745_06610) 1246691..1247451 + 761 Protein_1245 phage portal protein -
NG745_RS06615 (NG745_06615) 1247503..1247766 + 264 WP_098081338.1 hemolysin XhlA family protein -
NG745_RS06620 (NG745_06620) 1247780..1248043 + 264 WP_003154813.1 phage holin -
NG745_RS06625 (NG745_06625) 1248057..1248935 + 879 WP_043021264.1 N-acetylmuramoyl-L-alanine amidase -
NG745_RS06635 (NG745_06635) 1249192..1249362 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
NG745_RS06640 (NG745_06640) 1249363..1250109 - 747 WP_032866111.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
NG745_RS06645 (NG745_06645) 1250214..1251212 - 999 WP_007407255.1 inorganic phosphate transporter -
NG745_RS06650 (NG745_06650) 1251225..1251842 - 618 WP_003154804.1 DUF47 domain-containing protein -
NG745_RS06655 (NG745_06655) 1252128..1253444 - 1317 WP_007610842.1 amino acid permease -
NG745_RS06660 (NG745_06660) 1253767..1254717 + 951 WP_098081339.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29095.60 Da        Isoelectric Point: 4.7755

>T248931 WP_032866111.1 NZ_CP099465:c1250109-1249363 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT248931 WP_003154807.1 NZ_CP099465:c1249362-1249192 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References