Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485683..486320 Replicon chromosome
Accession NZ_CP099465
Organism Bacillus velezensis strain Ag75

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NG745_RS02460 Protein ID WP_003156187.1
Coordinates 485970..486320 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NG745_RS02455 Protein ID WP_003156188.1
Coordinates 485683..485964 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NG745_RS02435 (NG745_02435) 482048..482647 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NG745_RS02440 (NG745_02440) 482740..483105 + 366 WP_007410229.1 holo-ACP synthase -
NG745_RS02445 (NG745_02445) 483270..484277 + 1008 WP_098081024.1 outer membrane lipoprotein carrier protein LolA -
NG745_RS02450 (NG745_02450) 484394..485563 + 1170 WP_098081026.1 alanine racemase -
NG745_RS02455 (NG745_02455) 485683..485964 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NG745_RS02460 (NG745_02460) 485970..486320 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NG745_RS02465 (NG745_02465) 486438..487259 + 822 WP_003156182.1 STAS domain-containing protein -
NG745_RS02470 (NG745_02470) 487264..487629 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NG745_RS02475 (NG745_02475) 487632..488033 + 402 WP_003156178.1 anti-sigma regulatory factor -
NG745_RS02480 (NG745_02480) 488045..489052 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
NG745_RS02485 (NG745_02485) 489116..489445 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NG745_RS02490 (NG745_02490) 489442..489924 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NG745_RS02495 (NG745_02495) 489890..490678 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NG745_RS02500 (NG745_02500) 490678..491280 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T248930 WP_003156187.1 NZ_CP099465:485970-486320 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT248930 WP_003156188.1 NZ_CP099465:485683-485964 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References