Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489827..490464 Replicon chromosome
Accession NZ_CP099460
Organism Bacillus velezensis strain E

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NF199_RS02520 Protein ID WP_003156187.1
Coordinates 490114..490464 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NF199_RS02515 Protein ID WP_003156188.1
Coordinates 489827..490108 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NF199_RS02495 486192..486791 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
NF199_RS02500 486884..487249 + 366 WP_007609580.1 holo-ACP synthase -
NF199_RS02505 487414..488421 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
NF199_RS02510 488538..489707 + 1170 WP_032873001.1 alanine racemase -
NF199_RS02515 489827..490108 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NF199_RS02520 490114..490464 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NF199_RS02525 490582..491403 + 822 WP_003156182.1 STAS domain-containing protein -
NF199_RS02530 491408..491773 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NF199_RS02535 491776..492177 + 402 WP_003156178.1 anti-sigma regulatory factor -
NF199_RS02540 492189..493196 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
NF199_RS02545 493260..493589 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NF199_RS02550 493586..494068 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NF199_RS02555 494034..494822 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
NF199_RS02560 494822..495424 + 603 WP_265606115.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T248910 WP_003156187.1 NZ_CP099460:490114-490464 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT248910 WP_003156188.1 NZ_CP099460:489827-490108 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References