Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1676754..1677422 Replicon chromosome
Accession NC_014494
Organism Streptococcus pneumoniae AP200

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag SPAP_RS08815 Protein ID WP_001132284.1
Coordinates 1677243..1677422 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag SPAP_RS08810 Protein ID WP_000961810.1
Coordinates 1676754..1677206 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SPAP_RS08785 1672646..1673389 - 744 WP_001188212.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
SPAP_RS08790 1673391..1674341 - 951 WP_000451151.1 50S ribosomal protein L11 methyltransferase -
SPAP_RS08795 1674477..1674905 - 429 WP_000418174.1 NUDIX hydrolase -
SPAP_RS08800 1674886..1675974 - 1089 WP_000719713.1 M50 family metallopeptidase -
SPAP_RS08805 1675993..1676463 - 471 WP_000257086.1 DUF3013 family protein -
SPAP_RS08810 1676754..1677206 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SPAP_RS08815 1677243..1677422 - 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
SPAP_RS08820 1677559..1677738 - 180 WP_001048906.1 hypothetical protein -
SPAP_RS08825 1677903..1678118 - 216 WP_075329187.1 hypothetical protein -
SPAP_RS08830 1678416..1679687 + 1272 WP_001113212.1 replication-associated recombination protein A -
SPAP_RS08840 1680232..1681551 - 1320 WP_000502556.1 glycoside hydrolase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T24884 WP_001132284.1 NC_014494:c1677422-1677243 [Streptococcus pneumoniae AP200]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T24884 NC_014494:c1677422-1677243 [Streptococcus pneumoniae AP200]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT24884 WP_000961810.1 NC_014494:c1677206-1676754 [Streptococcus pneumoniae AP200]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT24884 NC_014494:c1677206-1676754 [Streptococcus pneumoniae AP200]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGGCTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References