Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4164467..4165043 Replicon chromosome
Accession NZ_CP099386
Organism Citrobacter braakii strain RHB09-E4-C01

Toxin (Protein)


Gene name shpA Uniprot ID A0A2I8S420
Locus tag NFJ61_RS19960 Protein ID WP_049269953.1
Coordinates 4164756..4165043 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFJ61_RS19955 Protein ID WP_016155614.1
Coordinates 4164467..4164769 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ61_RS19940 (4160963) 4160963..4161157 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFJ61_RS19945 (4161170) 4161170..4162126 + 957 WP_104651993.1 GTPase -
NFJ61_RS19950 (4162313) 4162313..4164160 + 1848 WP_016155615.1 3'-5' exonuclease -
NFJ61_RS19955 (4164467) 4164467..4164769 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFJ61_RS19960 (4164756) 4164756..4165043 - 288 WP_049269953.1 BrnT family toxin Toxin
NFJ61_RS19965 (4165275) 4165275..4166441 + 1167 WP_019078303.1 restriction endonuclease -
NFJ61_RS19970 (4166537) 4166537..4166692 + 156 Protein_3910 type I restriction endonuclease subunit R -
NFJ61_RS19975 (4166673) 4166673..4166905 + 233 Protein_3911 SymE family type I addiction module toxin -
NFJ61_RS19980 (4167036) 4167036..4167527 - 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFJ61_RS19985 (4167528) 4167528..4168034 - 507 WP_047414535.1 hypothetical protein -
NFJ61_RS19990 (4168034) 4168034..4168465 - 432 WP_223952673.1 DUF2778 domain-containing protein -
NFJ61_RS19995 (4168671) 4168671..4169735 - 1065 WP_049041982.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11433.87 Da        Isoelectric Point: 8.6051

>T248819 WP_049269953.1 NZ_CP099386:c4165043-4164756 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESRFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248819 WP_016155614.1 NZ_CP099386:c4164769-4164467 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S420

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References