Detailed information of TA system TA024881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1323160..1323768
• Replicon: chromosome
• Accession: NC_014483
• Organism: Paenibacillus polymyxa E681

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: PPE_RS05895
• Protein ID: WP_007429335.1
• Coordinates: 1323418..1323768 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PPE_RS05890
• Protein ID: WP_170970806.1
• Coordinates: 1323160..1323411 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPE_RS05880	1320104..1321381	+	1278	WP_013309245.1	outer membrane lipoprotein carrier protein LolA	-
PPE_RS05885	1321640..1322836	+	1197	WP_013309246.1	alanine racemase	-
PPE_RS05890	1323160..1323411	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
PPE_RS05895	1323418..1323768	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PPE_RS05900	1323975..1326170	+	2196	WP_190845174.1	RNA-binding transcriptional accessory protein	-
PPE_RS24905	1326275..1326409	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
PPE_RS05905	1326554..1327066	+	513	WP_043882100.1	SprT family protein	-
PPE_RS05920	1327454..1328329	-	876	WP_013309249.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T24881 WP_007429335.1 NC_014483:1323418-1323768 [Paenibacillus polymyxa E681]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T24881 NC_014483:1323418-1323768 [Paenibacillus polymyxa E681]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCCATCACCGCCCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTAGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT24881 WP_170970806.1 NC_014483:1323160-1323411 [Paenibacillus polymyxa E681]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT24881 NC_014483:1323160-1323411 [Paenibacillus polymyxa E681]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATCAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1