Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4308139..4308715 Replicon chromosome
Accession NZ_CP099385
Organism Citrobacter braakii strain RHB09-SO-C07

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag NFJ63_RS20715 Protein ID WP_049282566.1
Coordinates 4308428..4308715 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFJ63_RS20710 Protein ID WP_016155614.1
Coordinates 4308139..4308441 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ63_RS20695 (NFJ63_20690) 4304634..4304828 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFJ63_RS20700 (NFJ63_20695) 4304841..4305797 + 957 WP_115191028.1 GTPase -
NFJ63_RS20705 (NFJ63_20700) 4305984..4307831 + 1848 WP_153689754.1 NERD domain-containing protein/DEAD/DEAH box helicase -
NFJ63_RS20710 (NFJ63_20705) 4308139..4308441 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFJ63_RS20715 (NFJ63_20710) 4308428..4308715 - 288 WP_049282566.1 BrnT family toxin Toxin
NFJ63_RS20720 (NFJ63_20715) 4308949..4310115 + 1167 WP_019078303.1 restriction endonuclease -
NFJ63_RS20725 (NFJ63_20720) 4310211..4310357 + 147 Protein_4065 type I restriction endonuclease subunit R -
NFJ63_RS20730 (NFJ63_20725) 4310287..4310604 + 318 Protein_4066 endoribonuclease SymE -
NFJ63_RS20735 (NFJ63_20730) 4310699..4311190 - 492 Protein_4067 type VI secretion system tube protein TssD -
NFJ63_RS20740 (NFJ63_20735) 4311191..4311697 - 507 WP_049282567.1 hypothetical protein -
NFJ63_RS20745 (NFJ63_20740) 4311697..4312128 - 432 WP_228522538.1 DUF2778 domain-containing protein -
NFJ63_RS20750 (NFJ63_20745) 4312334..4313398 - 1065 WP_153689755.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11350.73 Da        Isoelectric Point: 7.5237

>T248806 WP_049282566.1 NZ_CP099385:c4308715-4308428 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQDRYENSEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248806 WP_016155614.1 NZ_CP099385:c4308441-4308139 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References