Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4121330..4121906 Replicon chromosome
Accession NZ_CP099384
Organism Citrobacter braakii strain RHB13-SO-C07

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag NFJ78_RS19550 Protein ID WP_222186473.1
Coordinates 4121619..4121906 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFJ78_RS19545 Protein ID WP_016155614.1
Coordinates 4121330..4121632 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ78_RS19530 (4117825) 4117825..4118019 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFJ78_RS19535 (4118032) 4118032..4118988 + 957 WP_053389311.1 GTPase -
NFJ78_RS19540 (4119175) 4119175..4121022 + 1848 WP_137380245.1 NERD domain-containing protein/DEAD/DEAH box helicase -
NFJ78_RS19545 (4121330) 4121330..4121632 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFJ78_RS19550 (4121619) 4121619..4121906 - 288 WP_222186473.1 BrnT family toxin Toxin
NFJ78_RS19555 (4122137) 4122137..4123303 + 1167 WP_019078303.1 restriction endonuclease -
NFJ78_RS19560 (4123399) 4123399..4123545 + 147 Protein_3830 type I restriction endonuclease subunit R -
NFJ78_RS19565 (4123532) 4123532..4123792 + 261 Protein_3831 endoribonuclease SymE -
NFJ78_RS19570 (4123887) 4123887..4124378 - 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFJ78_RS19575 (4124379) 4124379..4124885 - 507 WP_016155610.1 hypothetical protein -
NFJ78_RS19580 (4124885) 4124885..4125316 - 432 WP_223952673.1 DUF2778 domain-containing protein -
NFJ78_RS19585 (4125522) 4125522..4126586 - 1065 WP_049041982.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11334.73 Da        Isoelectric Point: 7.5237

>T248794 WP_222186473.1 NZ_CP099384:c4121906-4121619 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGIRFEDAILVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248794 WP_016155614.1 NZ_CP099384:c4121632-4121330 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References