248784

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4963741..4964357 Replicon chromosome
Accession NZ_CP099382
Organism Citrobacter braakii strain RHB14-SO-C08

Toxin (Protein)


Gene name vapC Uniprot ID A0A2I8SCX0
Locus tag NFJ83_RS23775 Protein ID WP_049269424.1
Coordinates 4963741..4964115 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag NFJ83_RS23780 Protein ID WP_016155181.1
Coordinates 4964115..4964357 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ83_RS23760 (4961244) 4961244..4962146 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
NFJ83_RS23765 (4962143) 4962143..4962778 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFJ83_RS23770 (4962775) 4962775..4963704 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFJ83_RS23775 (4963741) 4963741..4964115 - 375 WP_049269424.1 type II toxin-antitoxin system VapC family toxin Toxin
NFJ83_RS23780 (4964115) 4964115..4964357 - 243 WP_016155181.1 CopG family transcriptional regulator Antitoxin
NFJ83_RS23785 (4964563) 4964563..4965471 + 909 WP_049269423.1 alpha/beta hydrolase -
NFJ83_RS23790 (4965536) 4965536..4965778 + 243 WP_016155179.1 type II toxin-antitoxin system ParD family antitoxin -
NFJ83_RS23795 (4965771) 4965771..4966058 + 288 WP_049269420.1 type II toxin-antitoxin system RelE/ParE family toxin -
NFJ83_RS23800 (4966064) 4966064..4967005 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NFJ83_RS23805 (4967050) 4967050..4967487 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
NFJ83_RS23810 (4967484) 4967484..4968356 - 873 WP_019077938.1 virulence factor BrkB family protein -
NFJ83_RS23815 (4968350) 4968350..4968879 - 530 Protein_4656 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13650.78 Da        Isoelectric Point: 8.5373

>T248784 WP_049269424.1 NZ_CP099382:c4964115-4963741 [Citrobacter braakii]
MATGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT248784 WP_016155181.1 NZ_CP099382:c4964357-4964115 [Citrobacter braakii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8SCX0

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References