Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4383200..4383776 Replicon chromosome
Accession NZ_CP099382
Organism Citrobacter braakii strain RHB14-SO-C08

Toxin (Protein)


Gene name shpA Uniprot ID A0A2I8S420
Locus tag NFJ83_RS21085 Protein ID WP_049269953.1
Coordinates 4383489..4383776 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFJ83_RS21080 Protein ID WP_016155614.1
Coordinates 4383200..4383502 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ83_RS21065 (4379695) 4379695..4379889 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFJ83_RS21070 (4379902) 4379902..4380858 + 957 WP_049040419.1 GTPase -
NFJ83_RS21075 (4381045) 4381045..4382892 + 1848 WP_101700343.1 3'-5' exonuclease -
NFJ83_RS21080 (4383200) 4383200..4383502 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFJ83_RS21085 (4383489) 4383489..4383776 - 288 WP_049269953.1 BrnT family toxin Toxin
NFJ83_RS21090 (4384008) 4384008..4385174 + 1167 WP_279265941.1 restriction endonuclease -
NFJ83_RS21095 (4385270) 4385270..4385425 + 156 Protein_4133 type I restriction endonuclease subunit R -
NFJ83_RS21100 (4385406) 4385406..4385638 + 233 Protein_4134 SymE family type I addiction module toxin -
NFJ83_RS21105 (4385769) 4385769..4386260 - 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFJ83_RS21110 (4386261) 4386261..4386767 - 507 WP_047414535.1 hypothetical protein -
NFJ83_RS21115 (4386767) 4386767..4387198 - 432 WP_233896696.1 DUF2778 domain-containing protein -
NFJ83_RS21120 (4387404) 4387404..4388468 - 1065 WP_049041982.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11433.87 Da        Isoelectric Point: 8.6051

>T248782 WP_049269953.1 NZ_CP099382:c4383776-4383489 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESRFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248782 WP_016155614.1 NZ_CP099382:c4383502-4383200 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S420

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References