24877

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 524899..525526 Replicon chromosome
Accession NC_014410
Organism Thermoanaerobacterium thermosaccharolyticum DSM 571

Toxin (Protein)


Gene name mazF Uniprot ID D9TST7
Locus tag TTHE_RS02730 Protein ID WP_013297077.1
Coordinates 525176..525526 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I3VUF9
Locus tag TTHE_RS02725 Protein ID WP_013297076.1
Coordinates 524899..525174 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TTHE_RS02705 520666..521028 + 363 WP_013297072.1 hypothetical protein -
TTHE_RS02710 521108..522634 + 1527 WP_013297073.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
TTHE_RS02715 522648..523631 + 984 WP_049774892.1 outer membrane lipoprotein carrier protein LolA -
TTHE_RS02720 523645..524811 + 1167 WP_013297075.1 alanine racemase -
TTHE_RS02725 524899..525174 + 276 WP_013297076.1 ribbon-helix-helix protein, CopG family Antitoxin
TTHE_RS02730 525176..525526 + 351 WP_013297077.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
TTHE_RS02735 525645..526091 + 447 WP_013297078.1 hypothetical protein -
TTHE_RS02740 526210..528231 + 2022 WP_013297079.1 hypothetical protein -
TTHE_RS02745 528278..529348 + 1071 WP_013297080.1 polysaccharide pyruvyl transferase CsaB -
TTHE_RS02750 529361..530092 + 732 WP_013297081.1 WecB/TagA/CpsF family glycosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12945.03 Da        Isoelectric Point: 6.4714

>T24877 WP_013297077.1 NC_014410:525176-525526 [Thermoanaerobacterium thermosaccharolyticum DSM 571]
MIIKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T24877 NC_014410:525176-525526 [Thermoanaerobacterium thermosaccharolyticum DSM 571]
ATGATTATAAAGCGTGGAGATATTTTATATGCTGATTTAAGCCCTGTAATTGGCTCAGAACAGGGTGGTGTTAGGCCCGT
ACTTGTTATACAAAACGACATCGGCAATAAGTACAGTCCTACAGTTATTGTATCGGCAATAACATCTCAAATAAACAAGG
CTAAATTGCCAACACATGTTGAGATAAATGGGACTGAATACGGACTTAATAAAGATTCTGTTATTTTGTTGGAACAAATT
AGGACTATAGATAAAAAGCGGCTTAGGGAAAAAATAGGTCATTTTGATCAAGAGATGATGGAAAAAGTAAATGAGGCGCT
GCAAATAAGCCTAGGGTTAATAGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10514.31 Da        Isoelectric Point: 7.7813

>AT24877 WP_013297076.1 NC_014410:524899-525174 [Thermoanaerobacterium thermosaccharolyticum DSM 571]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Download         Length: 276 bp

>AT24877 NC_014410:524899-525174 [Thermoanaerobacterium thermosaccharolyticum DSM 571]
GTGGGCGAGACAAAAAGAATACTCGTCAGTTTGCCGGAGAGCTTACTGGAAGAAGTTGATGTGCTTGCAGCTATGGAAAA
TAGAAACCGCAGTGAATTTATAAGAGAAGCTATGAAGCTGTACATCAAGGAAAGAAAGAAGATGAAAATAAGGGAGAGCA
TGAAAAAAGGGTACCTTGAGATGGCGGCAATAAATGCAGAACTTGCGGAATTGGGCCTAACCGCTGACAATGAATGTTTT
AACGGATATGAAAAAAAATTAAAAAAGTGTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231VMD9

Antitoxin

Source ID Structure
AlphaFold DB I3VUF9

References