Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 848006..848660 Replicon chromosome
Accession NZ_CP099381
Organism Citrobacter braakii strain RHB16-E2-C02

Toxin (Protein)


Gene name cptA Uniprot ID R8WN60
Locus tag NFJ84_RS04095 Protein ID WP_016154348.1
Coordinates 848253..848660 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID R8WMJ6
Locus tag NFJ84_RS04090 Protein ID WP_016154349.1
Coordinates 848006..848272 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ84_RS04065 (NFJ84_04055) 843219..844652 - 1434 WP_016154353.1 6-phospho-beta-glucosidase BglA -
NFJ84_RS04070 (NFJ84_04060) 844773..845501 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
NFJ84_RS04075 (NFJ84_04065) 845554..845865 + 312 WP_016154352.1 N(4)-acetylcytidine aminohydrolase -
NFJ84_RS04080 (NFJ84_04070) 846029..846688 + 660 WP_016154351.1 hemolysin III family protein -
NFJ84_RS04085 (NFJ84_04075) 846768..847748 - 981 WP_235117299.1 tRNA-modifying protein YgfZ -
NFJ84_RS04090 (NFJ84_04080) 848006..848272 + 267 WP_016154349.1 FAD assembly factor SdhE Antitoxin
NFJ84_RS04095 (NFJ84_04085) 848253..848660 + 408 WP_016154348.1 protein YgfX Toxin
NFJ84_RS04100 (NFJ84_04090) 848761..849282 - 522 WP_279265524.1 flavodoxin FldB -
NFJ84_RS04105 (NFJ84_04095) 849396..850292 + 897 WP_016154346.1 site-specific tyrosine recombinase XerD -
NFJ84_RS04110 (NFJ84_04100) 850316..851029 + 714 WP_103766660.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NFJ84_RS04115 (NFJ84_04105) 851035..852768 + 1734 WP_016157413.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15850.66 Da        Isoelectric Point: 11.2845

>T248764 WP_016154348.1 NZ_CP099381:848253-848660 [Citrobacter braakii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10557.10 Da        Isoelectric Point: 5.6375

>AT248764 WP_016154349.1 NZ_CP099381:848006-848272 [Citrobacter braakii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R8WN60

Antitoxin

Source ID Structure
AlphaFold DB R8WMJ6

References