Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4115130..4115706 Replicon chromosome
Accession NZ_CP099378
Organism Citrobacter braakii strain RHB21-E1-C04

Toxin (Protein)


Gene name shpA Uniprot ID A0A8I0KMP7
Locus tag NFK10_RS19840 Protein ID WP_016155613.1
Coordinates 4115419..4115706 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFK10_RS19835 Protein ID WP_016155614.1
Coordinates 4115130..4115432 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK10_RS19820 (4111632) 4111632..4111826 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFK10_RS19825 (4111839) 4111839..4112795 + 957 WP_016155616.1 GTPase -
NFK10_RS19830 (4112982) 4112982..4114829 + 1848 WP_016155615.1 3'-5' exonuclease -
NFK10_RS19835 (4115130) 4115130..4115432 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFK10_RS19840 (4115419) 4115419..4115706 - 288 WP_016155613.1 BrnT family toxin Toxin
NFK10_RS19845 (4115941) 4115941..4117107 + 1167 WP_019078303.1 restriction endonuclease -
NFK10_RS19850 (4117203) 4117203..4117349 + 147 Protein_3886 type I restriction endonuclease subunit R -
NFK10_RS19855 (4117336) 4117336..4117596 + 261 Protein_3887 endoribonuclease SymE -
NFK10_RS19860 (4117691) 4117691..4118182 - 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFK10_RS19865 (4118183) 4118183..4118689 - 507 WP_016155610.1 hypothetical protein -
NFK10_RS19870 (4118689) 4118689..4119120 - 432 WP_225836832.1 DUF2778 domain-containing protein -
NFK10_RS19875 (4119278) 4119278..4119448 - 171 Protein_3891 hypothetical protein -
NFK10_RS19880 (4119454) 4119454..4119908 - 455 Protein_3892 MarR family transcriptional regulator -
NFK10_RS19885 (4120057) 4120057..4120221 - 165 WP_016155606.1 DUF1127 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11310.75 Da        Isoelectric Point: 7.4687

>T248748 WP_016155613.1 NZ_CP099378:c4115706-4115419 [Citrobacter braakii]
MPMEFEWDANKAQSNLRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248748 WP_016155614.1 NZ_CP099378:c4115432-4115130 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References