Detailed information of TA system TA024872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 781246..781781
• Replicon: chromosome
• Accession: NC_014394
• Organism: Gallionella capsiferriformans ES-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D9SDL6
• Locus tag: GALF_RS03700
• Protein ID: WP_013292714.1
• Coordinates: 781506..781781 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D9SDL5
• Locus tag: GALF_RS03695
• Protein ID: WP_013292713.1
• Coordinates: 781246..781506 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GALF_RS03670	776314..776820	+	507	WP_013292708.1	hypothetical protein	-
GALF_RS03675	777006..778535	-	1530	WP_013292709.1	IS66 family transposase	-
GALF_RS03680	778593..778931	-	339	WP_013292710.1	IS66 family insertion sequence element accessory protein TnpB	-
GALF_RS03685	778928..779290	-	363	WP_013292711.1	IS66 family insertion sequence hypothetical protein	-
GALF_RS14790	779526..780866	-	1341	WP_013292712.1	integrase family protein	-
GALF_RS03695	781246..781506	+	261	WP_013292713.1	ribbon-helix-helix domain-containing protein	Antitoxin
GALF_RS03700	781506..781781	+	276	WP_013292714.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GALF_RS03705	781844..782065	+	222	WP_013292715.1	RNA-binding S4 domain-containing protein	-
GALF_RS03710	782215..782886	-	672	WP_013292716.1	Crp/Fnr family transcriptional regulator	-
GALF_RS03715	783008..783475	+	468	WP_013292717.1	cytochrome c	-
GALF_RS03720	783753..783980	-	228	WP_013292718.1	hypothetical protein	-
GALF_RS03725	784281..784610	+	330	WP_013292720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GALF_RS03730	784600..784908	+	309	WP_013292721.1	helix-turn-helix domain-containing protein	-
GALF_RS03735	785300..786301	+	1002	WP_013292722.1	NERD domain-containing protein	-
GALF_RS03740	786345..786644	+	300	WP_013292723.1	GIY-YIG nuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	714838..785098	70260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10698.38 Da
• Isoelectric Point: 9.6217

>T24872 WP_013292714.1 NC_014394:781506-781781 [Gallionella capsiferriformans ES-2]
MELKWTGKALSDLARLHEFLSPLNKQAAAATVQSLTAAPAKLMVQPRLGERLEEFDPREVRRILVGHYEMRYEIQHSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T24872 NC_014394:781506-781781 [Gallionella capsiferriformans ES-2]
ATGGAATTGAAATGGACGGGCAAGGCATTGTCAGATTTGGCAAGGCTGCATGAGTTTCTTTCACCATTAAACAAGCAAGC
CGCTGCCGCCACTGTGCAATCCCTGACGGCAGCACCCGCCAAGCTCATGGTGCAACCACGTCTGGGTGAAAGGCTGGAAG
AGTTTGATCCTCGTGAGGTGCGGCGTATTTTGGTTGGTCATTATGAGATGCGTTATGAAATTCAACATTCCACAATTTAC
CTGCTGAGACTCTGGCACACGCGGGAAGATCGTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9667.17 Da
• Isoelectric Point: 6.2134

>AT24872 WP_013292713.1 NC_014394:781246-781506 [Gallionella capsiferriformans ES-2]
MLTKVLTAHVPVPLIEKVDLIAARMERSRGWIVKQALSAWVDQEELRRRMTLDAMADVDAGHVIDHQSVQAWADSLSSDQ
PLPVPR

Nucleotide

• Length: 261 bp

>AT24872 NC_014394:781246-781506 [Gallionella capsiferriformans ES-2]
ATGCTCACTAAAGTGCTCACAGCGCATGTTCCCGTTCCACTCATTGAAAAGGTTGATTTAATCGCCGCTCGCATGGAACG
TTCTCGCGGATGGATCGTGAAGCAGGCGCTGTCCGCTTGGGTTGATCAGGAAGAATTGCGTCGTCGTATGACACTGGACG
CGATGGCAGATGTGGATGCGGGTCACGTCATCGACCATCAATCCGTGCAAGCTTGGGCGGACAGTCTTAGTAGCGATCAA
CCGCTGCCGGTGCCGCGTTGA

Structures

Toxin

• AlphaFold DB: D9SDL6

Antitoxin

• AlphaFold DB: D9SDL5