248714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4886310..4886926 Replicon chromosome
Accession NZ_CP099375
Organism Citrobacter braakii strain RHB25-E4-C05

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag NFK28_RS23445 Protein ID WP_053388922.1
Coordinates 4886310..4886684 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag NFK28_RS23450 Protein ID WP_016155181.1
Coordinates 4886684..4886926 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK28_RS23430 (4883813) 4883813..4884715 + 903 WP_053388921.1 formate dehydrogenase O subunit beta -
NFK28_RS23435 (4884712) 4884712..4885347 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NFK28_RS23440 (4885344) 4885344..4886273 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NFK28_RS23445 (4886310) 4886310..4886684 - 375 WP_053388922.1 type II toxin-antitoxin system VapC family toxin Toxin
NFK28_RS23450 (4886684) 4886684..4886926 - 243 WP_016155181.1 CopG family transcriptional regulator Antitoxin
NFK28_RS23455 (4887131) 4887131..4888060 + 930 WP_149330683.1 alpha/beta hydrolase -
NFK28_RS23460 (4888145) 4888145..4888453 + 309 WP_053388923.1 type II toxin-antitoxin system HigB family toxin -
NFK28_RS23465 (4888453) 4888453..4888905 + 453 WP_053388924.1 helix-turn-helix domain-containing protein -
NFK28_RS23470 (4888923) 4888923..4889864 - 942 WP_053388925.1 fatty acid biosynthesis protein FabY -
NFK28_RS23475 (4889909) 4889909..4890346 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
NFK28_RS23480 (4890343) 4890343..4891215 - 873 WP_019077938.1 virulence factor BrkB family protein -
NFK28_RS23485 (4891209) 4891209..4891808 - 600 WP_016157873.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13738.93 Da        Isoelectric Point: 8.5373

>T248714 WP_053388922.1 NZ_CP099375:c4886684-4886310 [Citrobacter braakii]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQEFRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT248714 WP_016155181.1 NZ_CP099375:c4886926-4886684 [Citrobacter braakii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References