Detailed information of TA system TA024869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 2196218..2196825
• Replicon: chromosome
• Accession: NC_014378
• Organism: Acetohalobium arabaticum DSM 5501

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: D9QT85
• Locus tag: acear_RS10645
• Protein ID: WP_013279028.1
• Coordinates: 2196218..2196568 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: acear_RS10650
• Protein ID: WP_041667735.1
• Coordinates: 2196571..2196825 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
acear_RS10625	2191629..2192102	-	474	WP_013279024.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
acear_RS10630	2192193..2192699	-	507	WP_013279025.1	ECF transporter S component	-
acear_RS10635	2192720..2194909	-	2190	WP_013279026.1	tetratricopeptide repeat protein	-
acear_RS10640	2194935..2196134	-	1200	WP_013279027.1	amidohydrolase	-
acear_RS10645	2196218..2196568	-	351	WP_013279028.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
acear_RS10650	2196571..2196825	-	255	WP_041667735.1	ribbon-helix-helix protein, CopG family	Antitoxin
acear_RS12640	2197053..2197226	-	174	WP_013279030.1	hypothetical protein	-
acear_RS10655	2197361..2199604	-	2244	WP_013279031.1	IGHMBP2 family helicase	-
acear_RS10660	2199709..2200374	-	666	WP_013279032.1	isoprenoid biosynthesis glyoxalase ElbB	-
acear_RS10665	2200385..2200831	-	447	WP_013279033.1	CBS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12890.85 Da
• Isoelectric Point: 6.2341

>T24869 WP_013279028.1 NC_014378:c2196568-2196218 [Acetohalobium arabaticum DSM 5501]
VAIKRGDVFYADLNPVVGSEQGGIRPVLVIQNDIGNKYSPTVIVAAITSKIKKGKLPTHVEIDSNEHNLDKDSVILLEQI
RTIDKKRLRRQVTHLNEEIVKDVDEALEISVGLIDL

Nucleotide

• Length: 351 bp

>T24869 NC_014378:c2196568-2196218 [Acetohalobium arabaticum DSM 5501]
GTGGCTATCAAACGAGGCGATGTATTTTATGCAGATTTGAATCCGGTAGTCGGTTCTGAACAGGGAGGAATAAGACCGGT
ATTAGTAATTCAGAATGATATAGGTAATAAATATAGTCCTACTGTAATTGTGGCAGCCATTACTTCTAAGATCAAGAAAG
GAAAACTGCCTACTCATGTAGAGATAGACTCTAATGAGCATAATTTAGATAAGGATTCAGTAATTCTTTTAGAACAGATT
AGAACCATTGATAAGAAGCGGCTGCGCAGGCAGGTTACTCATTTAAATGAAGAGATAGTTAAAGATGTAGATGAAGCTTT
AGAGATTAGCGTTGGTCTAATCGATTTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9854.14 Da
• Isoelectric Point: 4.3103

>AT24869 WP_041667735.1 NC_014378:c2196825-2196571 [Acetohalobium arabaticum DSM 5501]
MISLPDSLLEKVDGVAEQENQNRSEFVRKAMKLYIAELRKEEIKQEMKQGYLEMGNINLEIAEDNIRIENRDFSKYEANI
AECE

Nucleotide

• Length: 255 bp

>AT24869 NC_014378:c2196825-2196571 [Acetohalobium arabaticum DSM 5501]
ATGATTAGCTTGCCGGATAGTTTATTAGAAAAGGTAGATGGGGTTGCCGAACAAGAGAATCAAAACCGCAGTGAGTTTGT
GCGTAAAGCTATGAAGCTCTATATTGCTGAGCTGCGTAAAGAAGAGATTAAACAGGAGATGAAGCAGGGCTATTTAGAAA
TGGGGAATATTAATTTAGAAATAGCAGAAGATAATATCAGGATAGAGAATAGAGACTTTTCAAAGTATGAAGCTAATATT
GCGGAGTGTGAATAG

Structures

Toxin

• AlphaFold DB: D9QT85