Detailed information of TA system TA024868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1921711..1922338
• Replicon: chromosome
• Accession: NC_014377
• Organism: Thermosediminibacter oceani DSM 16646

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: D9RZ88
• Locus tag: TOCE_RS09625
• Protein ID: WP_013276663.1
• Coordinates: 1921711..1922061 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D9RZ89
• Locus tag: TOCE_RS09630
• Protein ID: WP_013276664.1
• Coordinates: 1922063..1922338 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOCE_RS09605	1917522..1918679	-	1158	WP_013276659.1	amidohydrolase	-
TOCE_RS09610	1918774..1919592	-	819	WP_013276660.1	M55 family metallopeptidase	-
TOCE_RS09615	1919650..1920360	-	711	WP_013276661.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
TOCE_RS09620	1920362..1921597	-	1236	WP_013276662.1	HD-GYP domain-containing protein	-
TOCE_RS09625	1921711..1922061	-	351	WP_013276663.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TOCE_RS09630	1922063..1922338	-	276	WP_013276664.1	ribbon-helix-helix protein, CopG family	Antitoxin
TOCE_RS09635	1922399..1923574	-	1176	WP_013276665.1	alanine racemase	-
TOCE_RS09640	1923537..1925150	-	1614	WP_013276666.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
TOCE_RS09645	1925110..1925526	-	417	WP_013276667.1	holo-ACP synthase	-
TOCE_RS09650	1925743..1926177	+	435	WP_013276668.1	Hsp20 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12974.05 Da
• Isoelectric Point: 7.0147

>T24868 WP_013276663.1 NC_014377:c1922061-1921711 [Thermosediminibacter oceani DSM 16646]
VLVRRGDVFYADLNPVVGSEQGGVRPVLVVQNDVGNKYSPTVIVAAITSQIDKAKLPTHVELKRGEYGLEKDSVILLEQL
RTIDKRRLREKIAYLDQEIMARVDEALKISLGLVDF

Nucleotide

• Length: 351 bp

>T24868 NC_014377:c1922061-1921711 [Thermosediminibacter oceani DSM 16646]
GTGCTGGTAAGGCGCGGAGATGTGTTCTATGCGGATTTAAACCCGGTTGTGGGTTCCGAACAGGGCGGTGTAAGGCCCGT
CCTCGTTGTGCAAAACGACGTGGGAAATAAGTACAGCCCTACCGTAATTGTTGCTGCAATTACGTCCCAGATTGACAAGG
CCAAGCTGCCGACCCACGTGGAGCTAAAGCGGGGAGAATACGGCCTGGAAAAGGATTCGGTCATTCTTTTAGAACAGCTC
AGGACCATCGACAAAAGGCGATTGAGGGAGAAGATAGCTTACCTAGACCAGGAAATTATGGCAAGAGTAGATGAAGCGCT
GAAAATAAGCCTGGGACTGGTGGACTTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10830.44 Da
• Isoelectric Point: 5.2690

>AT24868 WP_013276664.1 NC_014377:c1922338-1922063 [Thermosediminibacter oceani DSM 16646]
VAELKRIVVSLPDSLLKQVDGIISKEKKNRSEFIQDAMRLYIRERERIRMREQLKNGYQEMADINLRIAEFGINEDFRDL
ALYEIRLSESD

Nucleotide

• Length: 276 bp

>AT24868 NC_014377:c1922338-1922063 [Thermosediminibacter oceani DSM 16646]
GTGGCTGAGTTAAAAAGGATAGTCGTCAGCTTACCGGATAGCCTGTTAAAGCAGGTGGATGGCATAATATCTAAGGAAAA
AAAGAACCGCAGCGAATTTATTCAGGATGCGATGAGATTATATATTAGAGAAAGGGAAAGGATAAGAATGAGAGAACAGC
TCAAAAACGGTTACCAGGAAATGGCCGATATAAACCTTAGGATCGCTGAATTCGGGATAAACGAGGATTTCAGAGATCTT
GCGCTTTACGAGATAAGGCTTTCGGAGAGTGACTAA

Structures

Toxin

• AlphaFold DB: D9RZ88

Antitoxin

• AlphaFold DB: D9RZ89