Detailed information of TA system TA024867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 531659..532257
• Replicon: chromosome
• Accession: NC_014377
• Organism: Thermosediminibacter oceani DSM 16646

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D9S1L8
• Locus tag: TOCE_RS02625
• Protein ID: WP_013275344.1
• Coordinates: 531659..531988 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D9S1L9
• Locus tag: TOCE_RS02630
• Protein ID: WP_013275345.1
• Coordinates: 531988..532257 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOCE_RS02615	529323..530369	-	1047	Protein_518	IS110 family transposase	-
TOCE_RS02620	530508..531602	+	1095	WP_083768457.1	P1 family peptidase	-
TOCE_RS02625	531659..531988	-	330	WP_013275344.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TOCE_RS02630	531988..532257	-	270	WP_013275345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TOCE_RS02635	532470..532862	+	393	WP_013275346.1	HEPN domain-containing protein	-
TOCE_RS02640	532816..533178	+	363	WP_013275347.1	nucleotidyltransferase domain-containing protein	-
TOCE_RS02645	533390..535756	+	2367	WP_013275348.1	type III-A CRISPR-associated protein Cas10/Csm1	-
TOCE_RS02650	535770..536192	+	423	WP_013275349.1	type III-A CRISPR-associated protein Csm2	-
TOCE_RS02655	536193..537026	+	834	WP_041423836.1	type III-A CRISPR-associated RAMP protein Csm3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (17-61)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12089.92 Da
• Isoelectric Point: 7.8599

>T24867 WP_013275344.1 NC_014377:c531988-531659 [Thermosediminibacter oceani DSM 16646]
MQAPDRGDLVYVNFNPQSGHEQAGRRPGIVLSPKPFNQLTGFAVFCPITRQKKGYPFEVELPVGLAVQGVILADQVKSLD
WRARQLQIVGRASNEIVSECLDLIHTFLS

Nucleotide

• Length: 330 bp

>T24867 NC_014377:c531988-531659 [Thermosediminibacter oceani DSM 16646]
ATGCAAGCGCCGGATCGCGGGGATCTCGTTTATGTGAATTTCAATCCACAATCAGGGCATGAACAAGCAGGAAGAAGGCC
GGGGATTGTTCTATCACCGAAACCATTTAATCAATTAACAGGATTTGCGGTTTTTTGCCCCATCACCCGGCAAAAAAAAG
GCTATCCGTTTGAAGTGGAATTACCAGTAGGCTTGGCTGTTCAAGGCGTGATTTTAGCTGATCAGGTCAAAAGTTTAGAT
TGGCGCGCTCGGCAGCTTCAAATAGTAGGACGGGCATCCAATGAAATTGTTTCGGAGTGCTTGGATCTGATTCATACTTT
TCTGTCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9989.69 Da
• Isoelectric Point: 6.4725

>AT24867 WP_013275345.1 NC_014377:c532257-531988 [Thermosediminibacter oceani DSM 16646]
MELNEPKGVPLMTAKVQKWGNSLAIRIPSVVAERLGIHQGSEMEMIIENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Nucleotide

• Length: 270 bp

>AT24867 NC_014377:c532257-531988 [Thermosediminibacter oceani DSM 16646]
ATGGAATTAAACGAACCAAAAGGGGTGCCTCTTATGACAGCAAAGGTGCAAAAATGGGGAAACAGCCTTGCGATCCGTAT
TCCAAGCGTGGTTGCTGAACGGCTTGGGATTCACCAAGGTTCAGAAATGGAAATGATTATTGAGAATCAGGCGATCAAGT
TGATCCCAAAAAAGAAAAAACCGACATTAGAAGAACTTTTGGCCAAAATAACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Structures

Toxin

• AlphaFold DB: D9S1L8

Antitoxin

• AlphaFold DB: D9S1L9