Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1218948..1219568 Replicon chromosome
Accession NZ_CP099366
Organism Citrobacter braakii strain RHB41-E2-C02

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag NFK67_RS05740 Protein ID WP_002892050.1
Coordinates 1218948..1219166 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag NFK67_RS05745 Protein ID WP_019076175.1
Coordinates 1219194..1219568 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK67_RS05705 (NFK67_05700) 1214307..1214936 + 630 WP_016155872.1 membrane protein -
NFK67_RS05710 (NFK67_05705) 1215001..1215354 + 354 WP_003831033.1 DUF1428 family protein -
NFK67_RS05715 (NFK67_05710) 1215406..1216959 - 1554 WP_279267474.1 EAL domain-containing protein -
NFK67_RS05720 (NFK67_05715) 1217148..1217408 + 261 WP_016152070.1 type B 50S ribosomal protein L31 -
NFK67_RS05725 (NFK67_05720) 1217410..1217550 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
NFK67_RS05730 (NFK67_05725) 1217629..1218099 - 471 WP_016152071.1 YlaC family protein -
NFK67_RS05735 (NFK67_05730) 1218216..1218767 - 552 WP_016155874.1 maltose O-acetyltransferase -
NFK67_RS05740 (NFK67_05735) 1218948..1219166 - 219 WP_002892050.1 HHA domain-containing protein Toxin
NFK67_RS05745 (NFK67_05740) 1219194..1219568 - 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
NFK67_RS05750 (NFK67_05745) 1220056..1223205 - 3150 WP_016152073.1 efflux RND transporter permease AcrB -
NFK67_RS05755 (NFK67_05750) 1223228..1224421 - 1194 WP_279267475.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T248646 WP_002892050.1 NZ_CP099366:c1219166-1218948 [Citrobacter braakii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT248646 WP_019076175.1 NZ_CP099366:c1219568-1219194 [Citrobacter braakii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References