Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 678613..679189 Replicon chromosome
Accession NZ_CP099366
Organism Citrobacter braakii strain RHB41-E2-C02

Toxin (Protein)


Gene name shpA Uniprot ID A0A2I8S420
Locus tag NFK67_RS03300 Protein ID WP_049269953.1
Coordinates 678613..678900 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFK67_RS03305 Protein ID WP_016155614.1
Coordinates 678887..679189 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK67_RS03265 (NFK67_03260) 673921..674985 + 1065 WP_049041982.1 DUF2955 domain-containing protein -
NFK67_RS03270 (NFK67_03265) 675191..675622 + 432 WP_223952673.1 DUF2778 domain-containing protein -
NFK67_RS03275 (NFK67_03270) 675622..676128 + 507 WP_047414535.1 hypothetical protein -
NFK67_RS03280 (NFK67_03275) 676129..676620 + 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFK67_RS03285 (NFK67_03280) 676751..676983 - 233 Protein_644 SymE family type I addiction module toxin -
NFK67_RS03290 (NFK67_03285) 676964..677119 - 156 Protein_645 type I restriction endonuclease subunit R -
NFK67_RS03295 (NFK67_03290) 677215..678381 - 1167 WP_279267423.1 restriction endonuclease -
NFK67_RS03300 (NFK67_03295) 678613..678900 + 288 WP_049269953.1 BrnT family toxin Toxin
NFK67_RS03305 (NFK67_03300) 678887..679189 + 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFK67_RS03310 (NFK67_03305) 679496..681343 - 1848 WP_137380245.1 NERD domain-containing protein/DEAD/DEAH box helicase -
NFK67_RS03315 (NFK67_03310) 681530..682486 - 957 WP_279267424.1 GTPase -
NFK67_RS03320 (NFK67_03315) 682499..682693 - 195 WP_003830038.1 YbdD/YjiX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11433.87 Da        Isoelectric Point: 8.6051

>T248645 WP_049269953.1 NZ_CP099366:678613-678900 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQERYENGEYRWQTIGLVHGIVVILVAHSVRFESRFEVIRIIS
ARKADRKERNRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248645 WP_016155614.1 NZ_CP099366:678887-679189 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S420

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References