Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 50094..50897 Replicon plasmid unnamed1
Accession NZ_CP099365
Organism Citrobacter braakii strain RHB44-SE-C08

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag NFL11_RS24100 Protein ID WP_016479963.1
Coordinates 50094..50624 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag NFL11_RS24105 Protein ID WP_022652312.1
Coordinates 50628..50897 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFL11_RS24075 (NFL11_24060) 46156..47692 - 1537 Protein_54 IS66 family transposase -
NFL11_RS24080 (NFL11_24065) 47741..48088 - 348 WP_004092452.1 IS66 family insertion sequence element accessory protein TnpB -
NFL11_RS24085 (NFL11_24070) 48085..48486 - 402 WP_004092453.1 transposase -
NFL11_RS24090 (NFL11_24075) 48648..48908 + 261 WP_063091378.1 hypothetical protein -
NFL11_RS24095 (NFL11_24080) 48905..49891 + 987 WP_279268736.1 hypothetical protein -
NFL11_RS24100 (NFL11_24085) 50094..50624 - 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
NFL11_RS24105 (NFL11_24090) 50628..50897 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
NFL11_RS24110 (NFL11_24095) 51231..52136 + 906 WP_032072928.1 RepB family plasmid replication initiator protein -
NFL11_RS24115 (NFL11_24100) 52462..53202 - 741 WP_104772627.1 tyrosine-type recombinase/integrase -
NFL11_RS24125 (NFL11_24110) 55204..55464 + 261 Protein_64 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ugd 1..91254 91254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T248643 WP_016479963.1 NZ_CP099365:c50624-50094 [Citrobacter braakii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT248643 WP_022652312.1 NZ_CP099365:c50897-50628 [Citrobacter braakii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References