Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4190101..4190677 Replicon chromosome
Accession NZ_CP099364
Organism Citrobacter braakii strain RHB44-SE-C08

Toxin (Protein)


Gene name shpA Uniprot ID A0A7L6U8Z2
Locus tag NFL11_RS20520 Protein ID WP_049040418.1
Coordinates 4190390..4190677 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2I8S1U6
Locus tag NFL11_RS20515 Protein ID WP_016155614.1
Coordinates 4190101..4190403 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFL11_RS20500 (4186596) 4186596..4186790 + 195 WP_003830038.1 YbdD/YjiX family protein -
NFL11_RS20505 (4186803) 4186803..4187759 + 957 WP_016155616.1 GTPase -
NFL11_RS20510 (4187946) 4187946..4189793 + 1848 WP_101700343.1 3'-5' exonuclease -
NFL11_RS20515 (4190101) 4190101..4190403 - 303 WP_016155614.1 BrnA antitoxin family protein Antitoxin
NFL11_RS20520 (4190390) 4190390..4190677 - 288 WP_049040418.1 BrnT family toxin Toxin
NFL11_RS20525 (4190911) 4190911..4192077 + 1167 WP_019078303.1 restriction endonuclease -
NFL11_RS20530 (4192173) 4192173..4192319 + 147 Protein_4022 type I restriction endonuclease subunit R -
NFL11_RS20535 (4192306) 4192306..4192566 + 261 Protein_4023 endoribonuclease SymE -
NFL11_RS20540 (4192661) 4192661..4193152 - 492 WP_016151575.1 type VI secretion system tube protein TssD -
NFL11_RS20545 (4193153) 4193153..4193659 - 507 WP_016155610.1 hypothetical protein -
NFL11_RS20550 (4193659) 4193659..4194090 - 432 WP_225836832.1 DUF2778 domain-containing protein -
NFL11_RS20555 (4194248) 4194248..4194418 - 171 Protein_4027 hypothetical protein -
NFL11_RS20560 (4194424) 4194424..4194878 - 455 Protein_4028 MarR family transcriptional regulator -
NFL11_RS20565 (4195027) 4195027..4195191 - 165 WP_016155606.1 DUF1127 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(42-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11293.68 Da        Isoelectric Point: 7.5237

>T248638 WP_049040418.1 NZ_CP099364:c4190677-4190390 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERSRYEHS

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11466.12 Da        Isoelectric Point: 10.1293

>AT248638 WP_016155614.1 NZ_CP099364:c4190403-4190101 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6U8Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S1U6

References