Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3815317..3815893 Replicon chromosome
Accession NZ_CP099358
Organism Escherichia fergusonii strain RHB02-E1-C03

Toxin (Protein)


Gene name shpA Uniprot ID A0A7W3EF74
Locus tag NFJ18_RS18490 Protein ID WP_024256534.1
Coordinates 3815606..3815893 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NFJ18_RS18485 Protein ID WP_000063156.1
Coordinates 3815317..3815619 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ18_RS18465 (3811131) 3811131..3811643 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
NFJ18_RS18470 (3811913) 3811913..3814063 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
NFJ18_RS18475 (3814113) 3814113..3814316 + 204 WP_000467859.1 YbdD/YjiX family protein -
NFJ18_RS18480 (3814327) 3814327..3815283 + 957 WP_001355122.1 GTPase -
NFJ18_RS18485 (3815317) 3815317..3815619 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NFJ18_RS18490 (3815606) 3815606..3815893 - 288 WP_024256534.1 BrnT family toxin Toxin
NFJ18_RS18495 (3816049) 3816049..3816783 - 735 WP_000208601.1 Fic family protein -
NFJ18_RS18500 (3817046) 3817046..3820558 + 3513 WP_181202857.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11241.74 Da        Isoelectric Point: 7.4020

>T248619 WP_024256534.1 NZ_CP099358:c3815893-3815606 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT248619 WP_000063156.1 NZ_CP099358:c3815619-3815317 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3EF74

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References