Detailed information of TA system TA024858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 3623947..3624454
• Replicon: chromosome
• Accession: NC_014364
• Organism: Spirochaeta smaragdinae DSM 11293

Toxin (Protein)

• Gene name: relB
• Uniprot ID: E1R2A4
• Locus tag: SPIRS_RS17025
• Protein ID: WP_013255923.1
• Coordinates: 3624194..3624454 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E1R2A3
• Locus tag: SPIRS_RS17020
• Protein ID: WP_013255922.1
• Coordinates: 3623947..3624201 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPIRS_RS22540	3619009..3619161	+	153	WP_013255917.1	hypothetical protein	-
SPIRS_RS16990	3619257..3619562	+	306	WP_013255918.1	nucleotidyltransferase domain-containing protein	-
SPIRS_RS16995	3619603..3619899	-	297	WP_041866118.1	hypothetical protein	-
SPIRS_RS17000	3619969..3621204	-	1236	WP_013255919.1	benzoate/H(+) symporter BenE family transporter	-
SPIRS_RS17005	3621280..3622254	-	975	WP_013255920.1	dihydrodipicolinate synthase family protein	-
SPIRS_RS17010	3622546..3623226	+	681	WP_013255921.1	GntR family transcriptional regulator	-
SPIRS_RS17015	3623472..3623816	+	345	WP_171814781.1	ImmA/IrrE family metallo-endopeptidase	-
SPIRS_RS17020	3623947..3624201	+	255	WP_013255922.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SPIRS_RS17025	3624194..3624454	+	261	WP_013255923.1	Txe/YoeB family addiction module toxin	Toxin
SPIRS_RS17030	3624757..3626217	-	1461	WP_013255924.1	FAD-dependent oxidoreductase	-
SPIRS_RS17035	3626247..3627959	-	1713	WP_013255925.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10244.38 Da
• Isoelectric Point: 6.7172

>T24858 WP_013255923.1 NC_014364:3624194-3624454 [Sediminispirochaeta smaragdinae DSM 11293]
MSRGVNWHESAWEDYEYWQGQDRKTLKKINTLIKTARRTPEEGAEPLKGDLSGYFSRHINKKDVLVYTYNDDSITIIQCR
FHYSDE

Nucleotide

• Length: 261 bp

>T24858 NC_014364:3624194-3624454 [Sediminispirochaeta smaragdinae DSM 11293]
ATGAGTAGAGGGGTCAACTGGCATGAGTCTGCATGGGAAGACTATGAGTACTGGCAAGGCCAAGACCGCAAAACACTGAA
GAAGATTAATACTTTAATAAAGACCGCACGACGAACGCCGGAAGAAGGAGCAGAACCCTTAAAGGGAGACCTCTCAGGTT
ACTTCTCCCGGCATATCAACAAGAAAGATGTACTGGTTTACACCTACAATGATGATTCGATCACCATTATTCAGTGCAGA
TTCCATTATTCTGATGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9478.70 Da
• Isoelectric Point: 5.7973

>AT24858 WP_013255922.1 NC_014364:3623947-3624201 [Sediminispirochaeta smaragdinae DSM 11293]
MRIMTYTNARNNLRQLIDDVVDTSTETVITSKEGRDVVVMSLADYNGWTTTNHLLSTPENASRLLKAARDVKAGRVIQRD
LIDE

Nucleotide

• Length: 255 bp

>AT24858 NC_014364:3623947-3624201 [Sediminispirochaeta smaragdinae DSM 11293]
ATGAGAATAATGACCTATACCAATGCGCGGAACAATCTCAGGCAACTGATTGATGACGTTGTAGATACCTCTACAGAAAC
TGTCATCACCTCAAAGGAAGGCCGAGATGTAGTGGTGATGTCCCTCGCAGATTACAATGGCTGGACAACCACCAACCACC
TGCTTAGTACACCTGAGAATGCCAGCCGCCTGTTAAAGGCTGCCCGCGACGTAAAGGCCGGGAGAGTTATCCAGCGAGAC
CTTATAGATGAGTAG

Structures

Toxin

• AlphaFold DB: E1R2A4

Antitoxin

• AlphaFold DB: E1R2A3