Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2122956..2123532 Replicon chromosome
Accession NZ_CP099349
Organism Escherichia fergusonii strain RHB16-E2-C07

Toxin (Protein)


Gene name shpA Uniprot ID A0A7W3EF74
Locus tag NFJ86_RS10435 Protein ID WP_024256534.1
Coordinates 2123245..2123532 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NFJ86_RS10430 Protein ID WP_000063156.1
Coordinates 2122956..2123258 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFJ86_RS10410 (2118770) 2118770..2119282 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
NFJ86_RS10415 (2119552) 2119552..2121702 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
NFJ86_RS10420 (2121752) 2121752..2121955 + 204 WP_000467859.1 YbdD/YjiX family protein -
NFJ86_RS10425 (2121966) 2121966..2122922 + 957 WP_001355122.1 GTPase -
NFJ86_RS10430 (2122956) 2122956..2123258 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NFJ86_RS10435 (2123245) 2123245..2123532 - 288 WP_024256534.1 BrnT family toxin Toxin
NFJ86_RS10440 (2123688) 2123688..2124422 - 735 WP_000208601.1 Fic family protein -
NFJ86_RS10445 (2124685) 2124685..2128197 + 3513 WP_181202857.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11241.74 Da        Isoelectric Point: 7.4020

>T248579 WP_024256534.1 NZ_CP099349:c2123532-2123245 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT248579 WP_000063156.1 NZ_CP099349:c2123258-2122956 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3EF74

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References