Detailed information of TA system TA024855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 730622..731187
• Replicon: chromosome
• Accession: NC_014364
• Organism: Spirochaeta smaragdinae DSM 11293

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SPIRS_RS03555
• Protein ID: WP_171814800.1
• Coordinates: 730622..730945 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: E1RBV8
• Locus tag: SPIRS_RS03560
• Protein ID: WP_013253302.1
• Coordinates: 730939..731187 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPIRS_RS03500	725785..726265	-	481	Protein_686	transposase	-
SPIRS_RS03505	726326..726994	+	669	WP_013253294.1	RICIN domain-containing protein	-
SPIRS_RS03510	727108..727332	-	225	WP_013253295.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SPIRS_RS03515	727333..727725	-	393	WP_013253296.1	type II toxin-antitoxin system VapC family toxin	-
SPIRS_RS22070	727710..727952	-	243	WP_083771448.1	transposase	-
SPIRS_RS03520	728069..728305	-	237	WP_049784581.1	putative toxin-antitoxin system toxin component, PIN family	-
SPIRS_RS03525	728309..728587	-	279	WP_013253297.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SPIRS_RS03535	728890..729198	+	309	WP_013253298.1	nucleotidyltransferase domain-containing protein	-
SPIRS_RS03540	729373..729615	+	243	WP_013253299.1	hypothetical protein	-
SPIRS_RS22080	729612..729824	+	213	WP_049784582.1	hypothetical protein	-
SPIRS_RS03550	730021..730443	-	423	WP_013253300.1	PIN domain-containing protein	-
SPIRS_RS03555	730622..730945	-	324	WP_171814800.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SPIRS_RS03560	730939..731187	-	249	WP_013253302.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SPIRS_RS03565	731245..732255	-	1011	WP_013253303.1	LacI family DNA-binding transcriptional regulator	-
SPIRS_RS03570	732490..733497	+	1008	WP_013253304.1	substrate-binding domain-containing protein	-
SPIRS_RS03575	733586..735088	+	1503	WP_013253305.1	sugar ABC transporter ATP-binding protein	-
SPIRS_RS03580	735085..736062	+	978	WP_013253306.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	727333..739552	12219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-105)

Antitoxin

• SpoVT-AbrB domain (8-48)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12266.75 Da
• Isoelectric Point: 10.4773

>T24855 WP_171814800.1 NC_014364:c730945-730622 [Sediminispirochaeta smaragdinae DSM 11293]
VVIAQYEVYLINLAPAVGHEIKKTRPCLIISPNEMNESIPTIIVAPMTTKSHPFPTRVPIVFEKKNGWIVLDQIRTVDRK
RLIKRIGKINKKEIVLVKNILQEMLVD

Nucleotide

• Length: 324 bp

>T24855 NC_014364:c730945-730622 [Sediminispirochaeta smaragdinae DSM 11293]
GTGGTAATAGCCCAATATGAAGTATATTTGATAAATTTGGCCCCTGCAGTAGGACATGAAATAAAAAAAACAAGGCCTTG
TTTGATAATTTCTCCCAATGAGATGAATGAATCAATCCCGACAATCATAGTAGCCCCTATGACAACAAAATCGCATCCCT
TTCCGACGCGTGTTCCCATTGTATTTGAAAAGAAAAATGGTTGGATAGTATTAGATCAGATAAGAACTGTTGATAGGAAA
AGACTAATTAAGAGAATTGGTAAGATCAATAAAAAAGAAATAGTACTTGTAAAAAATATACTGCAAGAGATGTTAGTAGA
TTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9713.08 Da
• Isoelectric Point: 4.4389

>AT24855 WP_013253302.1 NC_014364:c731187-730939 [Sediminispirochaeta smaragdinae DSM 11293]
MLVSVVPIGNSKGIRIPKSILQQLHIEEKVELEVHNKELLIRPIQRKPREGWSEKFMEMNNEGDDTLLIENVDEQDDFTW
EW

Nucleotide

• Length: 249 bp

>AT24855 NC_014364:c731187-730939 [Sediminispirochaeta smaragdinae DSM 11293]
ATGTTAGTTTCAGTTGTTCCTATAGGAAATTCAAAAGGCATACGGATTCCAAAGAGTATTTTACAACAGCTGCATATTGA
GGAGAAAGTAGAGCTGGAAGTCCATAATAAAGAACTACTGATTAGACCAATTCAAAGAAAACCAAGAGAAGGTTGGTCGG
AAAAGTTTATGGAGATGAACAATGAGGGAGATGATACCTTACTCATAGAGAATGTAGATGAGCAGGATGATTTTACATGG
GAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: E1RBV8