Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 251660..252302 Replicon chromosome
Accession NC_014335
Organism Bacillus cereus biovar anthracis str. CI

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BACI_RS01505 Protein ID WP_000635963.1
Coordinates 251952..252302 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BACI_RS01500 Protein ID WP_000004570.1
Coordinates 251660..251947 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BACI_RS01475 246976..247938 + 963 WP_000961153.1 UV DNA damage repair endonuclease UvsE -
BACI_RS01480 247931..248503 - 573 WP_000906927.1 rhomboid family intramembrane serine protease -
BACI_RS01485 248596..248955 + 360 WP_000635040.1 holo-ACP synthase -
BACI_RS01490 249112..250062 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BACI_RS01495 250181..251350 + 1170 WP_000390602.1 alanine racemase -
BACI_RS01500 251660..251947 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BACI_RS01505 251952..252302 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BACI_RS01510 252370..254538 + 2169 WP_000426221.1 RNA-binding transcriptional accessory protein -
BACI_RS01515 254596..254712 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BACI_RS01520 254908..255366 + 459 WP_000344246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T24852 WP_000635963.1 NC_014335:251952-252302 [Bacillus cereus biovar anthracis str. CI]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T24852 NC_014335:251952-252302 [Bacillus cereus biovar anthracis str. CI]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT24852 WP_000004570.1 NC_014335:251660-251947 [Bacillus cereus biovar anthracis str. CI]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT24852 NC_014335:251660-251947 [Bacillus cereus biovar anthracis str. CI]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References