Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 39494..40291 Replicon plasmid unnamed1
Accession NZ_CP099319
Organism Enterobacter hormaechei strain RHB45-SO-C02

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag NFL35_RS22910 Protein ID WP_006812498.1
Coordinates 39767..40291 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag NFL35_RS22905 Protein ID WP_006812497.1
Coordinates 39494..39763 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFL35_RS22880 (NFL35_22865) 34867..36207 - 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -
NFL35_RS22885 (NFL35_22870) 36268..36993 - 726 WP_006812583.1 hypothetical protein -
NFL35_RS22890 (NFL35_22875) 37257..38054 + 798 WP_006812584.1 DUF2971 domain-containing protein -
NFL35_RS22895 (NFL35_22880) 38096..38449 - 354 WP_160859104.1 hypothetical protein -
NFL35_RS22900 (NFL35_22885) 38455..39120 - 666 WP_023315962.1 AAA family ATPase -
NFL35_RS22905 (NFL35_22890) 39494..39763 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
NFL35_RS22910 (NFL35_22895) 39767..40291 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
NFL35_RS22915 (NFL35_22900) 40318..40482 - 165 WP_006812499.1 hypothetical protein -
NFL35_RS22920 (NFL35_22905) 40475..40726 - 252 WP_000147960.1 hypothetical protein -
NFL35_RS22925 (NFL35_22910) 40728..41420 - 693 WP_006812500.1 membrane protein -
NFL35_RS22930 (NFL35_22915) 41434..41757 - 324 WP_000064173.1 hypothetical protein -
NFL35_RS22935 (NFL35_22920) 41832..42620 - 789 WP_032655902.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..111406 111406


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T248438 WP_006812498.1 NZ_CP099319:39767-40291 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT248438 WP_006812497.1 NZ_CP099319:39494-39763 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References