Detailed information of TA system TA024843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 13959..14499
• Replicon: plasmid pNWAT01
• Accession: NC_014316
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D8KCE2
• Locus tag: NWAT_RS15380
• Protein ID: WP_004164066.1
• Coordinates: 14179..14499 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D8KCE1
• Locus tag: NWAT_RS15375
• Protein ID: WP_004164082.1
• Coordinates: 13959..14192 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS15340	9111..9632	-	522	WP_013221932.1	hypothetical protein	-
NWAT_RS15345	9742..10050	+	309	WP_013221933.1	hypothetical protein	-
NWAT_RS15350	10406..11173	+	768	WP_041351078.1	IncFII RepA protein family	-
NWAT_RS15355	11627..11962	+	336	WP_013221934.1	helix-turn-helix transcriptional regulator	-
NWAT_RS15360	12043..12279	+	237	WP_004164080.1	hypothetical protein	-
NWAT_RS15370	12921..13895	+	975	WP_198342225.1	ATP-binding protein	-
NWAT_RS15375	13959..14192	+	234	WP_004164082.1	hypothetical protein	Antitoxin
NWAT_RS15380	14179..14499	+	321	WP_004164066.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NWAT_RS15385	14503..15609	+	1107	WP_013221936.1	metal-dependent hydrolase	-
NWAT_RS15390	15690..17057	+	1368	WP_157680067.1	deoxyguanosinetriphosphate triphosphohydrolase	-
NWAT_RS15395	17331..17648	-	318	WP_013221938.1	hypothetical protein	-
NWAT_RS15400	17652..17993	-	342	WP_013221939.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39105	39105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11531.59 Da
• Isoelectric Point: 11.1935

>T24843 WP_004164066.1 NC_014316:14179-14499 [Nitrosococcus watsonii C-113]
MTRGEVYWVNLNPTQGSEIRKRRPCVLVGATPINQARRTVVVVPLSTSAKPRPPLTVPIQGLAQPVVAVCDQIRAVDKSR
LLEAAGRLSKKDLDALAEGLRQVLVL

Nucleotide

• Length: 321 bp

>T24843 NC_014316:14179-14499 [Nitrosococcus watsonii C-113]
ATGACTCGGGGTGAGGTGTATTGGGTGAATCTTAATCCCACCCAAGGGTCGGAAATCCGCAAGCGCCGCCCCTGCGTCTT
GGTGGGGGCCACGCCCATCAATCAGGCCCGGCGCACGGTAGTGGTCGTGCCCCTGTCCACCTCCGCCAAGCCCAGGCCGC
CCCTGACCGTGCCTATCCAGGGCCTCGCTCAGCCCGTGGTGGCGGTCTGCGATCAGATCCGTGCGGTGGATAAGTCCCGC
TTGCTGGAGGCAGCCGGCCGTTTATCCAAAAAAGACTTGGACGCTCTGGCGGAAGGGCTGAGGCAGGTGTTGGTCCTTTA
G

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8635.85 Da
• Isoelectric Point: 4.4217

>AT24843 WP_004164082.1 NC_014316:13959-14192 [Nitrosococcus watsonii C-113]
MQKVLFSLPPDLVARMRASIPPKQRSQVIARLLAQELEKREQALYQCALEVEADDALGEEMKDWDITVGDGLNHDSG

Nucleotide

• Length: 234 bp

>AT24843 NC_014316:13959-14192 [Nitrosococcus watsonii C-113]
ATGCAAAAAGTGCTCTTTTCCTTGCCCCCTGACTTGGTGGCGCGCATGCGGGCCAGCATCCCGCCGAAACAACGCAGCCA
GGTGATCGCCCGCCTCCTGGCGCAGGAGCTTGAGAAGCGGGAGCAGGCCCTTTATCAATGCGCCCTGGAAGTGGAGGCAG
ACGATGCCCTGGGGGAAGAAATGAAAGACTGGGACATCACCGTGGGCGATGGCCTCAACCATGACTCGGGGTGA

Structures

Toxin

• AlphaFold DB: D8KCE2

Antitoxin

• AlphaFold DB: D8KCE1