Detailed information of TA system TA248416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1090628..1092074
• Replicon: chromosome
• Accession: NZ_CP099316
• Organism: Enterobacter hormaechei strain RHB44-SE-C02

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: NFL06_RS05185
• Protein ID: WP_049086744.1
• Coordinates: 1091001..1092074 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: NFL06_RS05180
• Protein ID: WP_279262670.1
• Coordinates: 1090628..1091008 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NFL06_RS05155 (NFL06_05155)	1086301..1086972	+	672	WP_033486668.1	hypothetical protein	-
NFL06_RS05160 (NFL06_05160)	1086969..1088081	+	1113	WP_049086739.1	ImmA/IrrE family metallo-endopeptidase	-
NFL06_RS05165 (NFL06_05165)	1088205..1088414	-	210	WP_049086740.1	hypothetical protein	-
NFL06_RS05170 (NFL06_05170)	1088574..1089356	+	783	WP_049086741.1	hypothetical protein	-
NFL06_RS05175 (NFL06_05175)	1089546..1089914	-	369	WP_265196468.1	hypothetical protein	-
NFL06_RS05180 (NFL06_05180)	1090628..1091008	-	381	WP_279262670.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
NFL06_RS05185 (NFL06_05185)	1091001..1092074	-	1074	WP_049086744.1	type II toxin-antitoxin system RnlA family toxin	Toxin
NFL06_RS05190 (NFL06_05190)	1092812..1093396	+	585	WP_279262671.1	recombinase family protein	-
NFL06_RS05195 (NFL06_05195)	1093386..1093982	+	597	WP_279262672.1	hypothetical protein	-
NFL06_RS05200 (NFL06_05200)	1094317..1094466	-	150	WP_170975191.1	hypothetical protein	-
NFL06_RS05205 (NFL06_05205)	1094463..1094954	-	492	WP_279262673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1083612..1100752	17140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, DBD domain (203-325)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40502.49 Da
• Isoelectric Point: 5.8797

>T248416 WP_049086744.1 NZ_CP099316:c1092074-1091001 [Enterobacter hormaechei]
MTVRSYKDLNLVRANIETECRQFIQTRTYAIQSIGPMPGQKPGLRVTFAKTGENFATVNIFYNGDGSSTVQYLTGANQSL
GKELADYLFETINPEEFEHVNMVVEGFVEDSIFPVLELSAEETHIEFWEHLRNTHSIVWKITSTTYQDELTVTLYTTTGK
LQIQGRPLSCYRVFTFNLAELLNLQGLEKVLIRQDDGKANIVQQEVARANLESIMGDAYPHLHVTAEKLLISGLCVKLAA
PKLPDYCMLLYPELRSVEGVLKNKMYTFGMSVQQPLGFGPFFDKVGLNFILKPQYAAPLRPEQANAINEAYTFWNAERHA
VFHMETAVDSSRMISDMARLMTKATKAWQIIKDLYLV

Nucleotide

• Length: 1074 bp

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14019.16 Da
• Isoelectric Point: 4.3016

>AT248416 WP_279262670.1 NZ_CP099316:c1091008-1090628 [Enterobacter hormaechei]
MFEITGVNVSGSLKAVVMATGYENPLSSVNEIETKLTDLLGQNENGKILFDLLCANGLEWNRFVTLEMKYGRIMLDSAKI
IDVQEIPAQILSKLTFTLLNHPEYLEASVLTHDDVRQVLSMDMQGA

Nucleotide

• Length: 381 bp