Detailed information of TA system TA248403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1090629..1092075
• Replicon: chromosome
• Accession: NZ_CP099314
• Organism: Enterobacter hormaechei strain RHB44-SE-C01

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: NFL05_RS05180
• Protein ID: WP_049086744.1
• Coordinates: 1091002..1092075 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: NFL05_RS05175
• Protein ID: WP_279262670.1
• Coordinates: 1090629..1091009 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NFL05_RS05150 (NFL05_05150)	1086302..1086973	+	672	WP_033486668.1	hypothetical protein	-
NFL05_RS05155 (NFL05_05155)	1086970..1088082	+	1113	WP_049086739.1	ImmA/IrrE family metallo-endopeptidase	-
NFL05_RS05160 (NFL05_05160)	1088206..1088415	-	210	WP_049086740.1	hypothetical protein	-
NFL05_RS05165 (NFL05_05165)	1088575..1089357	+	783	WP_049086741.1	hypothetical protein	-
NFL05_RS05170 (NFL05_05170)	1089547..1089915	-	369	WP_265196468.1	hypothetical protein	-
NFL05_RS05175 (NFL05_05175)	1090629..1091009	-	381	WP_279262670.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
NFL05_RS05180 (NFL05_05180)	1091002..1092075	-	1074	WP_049086744.1	type II toxin-antitoxin system RnlA family toxin	Toxin
NFL05_RS05185 (NFL05_05185)	1092813..1093397	+	585	WP_279262671.1	recombinase family protein	-
NFL05_RS05190 (NFL05_05190)	1093387..1093983	+	597	WP_279262672.1	hypothetical protein	-
NFL05_RS05195 (NFL05_05195)	1094318..1094467	-	150	WP_170975191.1	hypothetical protein	-
NFL05_RS05200 (NFL05_05200)	1094464..1094955	-	492	WP_279262673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1083613..1100753	17140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, DBD domain (203-325)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40502.49 Da
• Isoelectric Point: 5.8797

>T248403 WP_049086744.1 NZ_CP099314:c1092075-1091002 [Enterobacter hormaechei]
MTVRSYKDLNLVRANIETECRQFIQTRTYAIQSIGPMPGQKPGLRVTFAKTGENFATVNIFYNGDGSSTVQYLTGANQSL
GKELADYLFETINPEEFEHVNMVVEGFVEDSIFPVLELSAEETHIEFWEHLRNTHSIVWKITSTTYQDELTVTLYTTTGK
LQIQGRPLSCYRVFTFNLAELLNLQGLEKVLIRQDDGKANIVQQEVARANLESIMGDAYPHLHVTAEKLLISGLCVKLAA
PKLPDYCMLLYPELRSVEGVLKNKMYTFGMSVQQPLGFGPFFDKVGLNFILKPQYAAPLRPEQANAINEAYTFWNAERHA
VFHMETAVDSSRMISDMARLMTKATKAWQIIKDLYLV

Nucleotide

• Length: 1074 bp

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14019.16 Da
• Isoelectric Point: 4.3016

>AT248403 WP_279262670.1 NZ_CP099314:c1091009-1090629 [Enterobacter hormaechei]
MFEITGVNVSGSLKAVVMATGYENPLSSVNEIETKLTDLLGQNENGKILFDLLCANGLEWNRFVTLEMKYGRIMLDSAKI
IDVQEIPAQILSKLTFTLLNHPEYLEASVLTHDDVRQVLSMDMQGA

Nucleotide

• Length: 381 bp