Detailed information of TA system TA248402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 617988..618564
• Replicon: chromosome
• Accession: NZ_CP099314
• Organism: Enterobacter hormaechei strain RHB44-SE-C01

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: NFL05_RS02955
• Protein ID: WP_015572580.1
• Coordinates: 617988..618275 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: NFL05_RS02960
• Protein ID: WP_017694570.1
• Coordinates: 618262..618564 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NFL05_RS02930 (NFL05_02930)	613076..613795	+	720	WP_017694573.1	winged helix-turn-helix domain-containing protein	-
NFL05_RS02935 (NFL05_02935)	613945..614415	+	471	WP_015572576.1	MarR family transcriptional regulator	-
NFL05_RS02940 (NFL05_02940)	614412..615479	+	1068	WP_026094404.1	HlyD family secretion protein	-
NFL05_RS02945 (NFL05_02945)	615469..616545	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
NFL05_RS02950 (NFL05_02950)	616542..617813	-	1272	WP_017694571.1	DUF445 domain-containing protein	-
NFL05_RS02955 (NFL05_02955)	617988..618275	+	288	WP_015572580.1	BrnT family toxin	Toxin
NFL05_RS02960 (NFL05_02960)	618262..618564	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
NFL05_RS02965 (NFL05_02965)	618593..619231	-	639	WP_017694569.1	LysE family translocator	-
NFL05_RS02970 (NFL05_02970)	619270..620022	-	753	WP_015572582.1	AraC family transcriptional regulator	-
NFL05_RS02975 (NFL05_02975)	620176..621546	+	1371	WP_017694568.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
NFL05_RS02980 (NFL05_02980)	621728..622273	-	546	WP_006810254.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T248402 WP_015572580.1 NZ_CP099314:617988-618275 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT248402 WP_017694570.1 NZ_CP099314:618262-618564 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp