Detailed information of TA system TA024839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2744191..2744604
• Replicon: chromosome
• Accession: NC_014315
• Organism: Nitrosococcus watsonii C-113

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D8K9Z0
• Locus tag: NWAT_RS12505
• Protein ID: WP_013221416.1
• Coordinates: 2744416..2744604 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D8K9Y9
• Locus tag: NWAT_RS12500
• Protein ID: WP_013221415.1
• Coordinates: 2744191..2744406 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NWAT_RS12455	2739634..2740080	-	447	Protein_2480	DUF2202 domain-containing protein	-
NWAT_RS12460	2740170..2740739	-	570	WP_013221408.1	NnrU family protein	-
NWAT_RS12465	2740758..2740973	-	216	WP_013221409.1	hypothetical protein	-
NWAT_RS16305	2741319..2741543	-	225	Protein_2483	DUF433 domain-containing protein	-
NWAT_RS12475	2741732..2741962	+	231	WP_013221410.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
NWAT_RS12480	2741959..2742294	+	336	WP_013221411.1	endoribonuclease MazF	-
NWAT_RS16310	2742427..2742618	-	192	WP_013221412.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NWAT_RS12485	2742605..2742805	-	201	WP_013221413.1	hypothetical protein	-
NWAT_RS12490	2742879..2743289	-	411	WP_013221414.1	hypothetical protein	-
NWAT_RS17115	2743656..2744105	-	450	WP_198342156.1	hypothetical protein	-
NWAT_RS12500	2744191..2744406	-	216	WP_013221415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NWAT_RS12505	2744416..2744604	-	189	WP_013221416.1	type II toxin-antitoxin system HicA family toxin	Toxin
NWAT_RS12510	2744717..2744935	-	219	WP_013221417.1	type II toxin-antitoxin system HicA family toxin	-
NWAT_RS12515	2744928..2745149	-	222	WP_013221418.1	type II toxin-antitoxin system HicB family antitoxin	-
NWAT_RS12520	2745470..2745841	-	372	WP_013221419.1	hypothetical protein	-
NWAT_RS12525	2746436..2748262	+	1827	WP_013221420.1	sodium:proton antiporter	-
NWAT_RS16785	2748522..2748680	+	159	WP_157679920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2733181..2745841	12660
	-	inside	Integron	-	-	2742605..2744707	2102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6845.18 Da
• Isoelectric Point: 11.2962

>T24839 WP_013221416.1 NC_014315:c2744604-2744416 [Nitrosococcus watsonii C-113]
MKVKELIKLIEADGWVQIRMKGSHRQFRHPTKVGIVTVSGKASVDIPVGTLKNALKQAGLHK

Nucleotide

• Length: 189 bp

>T24839 NC_014315:c2744604-2744416 [Nitrosococcus watsonii C-113]
ATGAAGGTCAAGGAGCTTATCAAGCTCATCGAAGCAGATGGTTGGGTACAAATTCGAATGAAAGGCAGTCACCGACAATT
CAGGCACCCGACCAAGGTGGGGATCGTAACAGTTTCCGGCAAGGCAAGTGTAGATATTCCAGTCGGCACATTGAAGAATG
CACTTAAACAAGCAGGGTTACACAAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.60 Da
• Isoelectric Point: 4.0737

>AT24839 WP_013221415.1 NC_014315:c2744406-2744191 [Nitrosococcus watsonii C-113]
MKYTVILEEGDSSYGAYVPDLPGCVAAGETREEALALIREAIEFHIEGLKQEGQPVPKPHSVSELVDVDTA

Nucleotide

• Length: 216 bp

>AT24839 NC_014315:c2744406-2744191 [Nitrosococcus watsonii C-113]
ATGAAATATACAGTCATTCTTGAGGAAGGCGACTCAAGCTACGGAGCATATGTTCCCGATCTGCCGGGTTGCGTAGCAGC
AGGAGAAACTCGCGAAGAAGCTTTAGCACTCATTAGAGAGGCAATCGAGTTCCACATTGAAGGATTGAAACAGGAAGGAC
AGCCTGTGCCGAAACCGCACAGCGTCAGCGAGCTTGTTGACGTGGATACCGCATAA

Structures

Toxin

• AlphaFold DB: D8K9Z0

Antitoxin

• AlphaFold DB: D8K9Y9