Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1018862..1019482 Replicon chromosome
Accession NZ_CP099309
Organism Enterobacter ludwigii strain RHB25-E3-C07

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag NFK27_RS04785 Protein ID WP_014168902.1
Coordinates 1018862..1019080 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag NFK27_RS04790 Protein ID WP_020885187.1
Coordinates 1019108..1019482 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NFK27_RS04755 (NFK27_04755) 1014873..1015133 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
NFK27_RS04760 (NFK27_04760) 1015136..1015276 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NFK27_RS04765 (NFK27_04765) 1015273..1015983 - 711 WP_181240139.1 GNAT family protein -
NFK27_RS04770 (NFK27_04770) 1016084..1017544 + 1461 WP_279260358.1 PLP-dependent aminotransferase family protein -
NFK27_RS04775 (NFK27_04775) 1017516..1017983 - 468 WP_032677533.1 YlaC family protein -
NFK27_RS04780 (NFK27_04780) 1018101..1018652 - 552 WP_020885188.1 maltose O-acetyltransferase -
NFK27_RS04785 (NFK27_04785) 1018862..1019080 - 219 WP_014168902.1 HHA domain-containing protein Toxin
NFK27_RS04790 (NFK27_04790) 1019108..1019482 - 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
NFK27_RS04795 (NFK27_04795) 1019994..1023140 - 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit AcrB -
NFK27_RS04800 (NFK27_04800) 1023163..1024356 - 1194 WP_020885186.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T248379 WP_014168902.1 NZ_CP099309:c1019080-1018862 [Enterobacter ludwigii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT248379 WP_020885187.1 NZ_CP099309:c1019482-1019108 [Enterobacter ludwigii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References